Please use this identifier to cite or link to this item: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/1591
Title: Modulation of Photophysics and pKa Shift of the Anti‐cancer Drug Camptothecin in the Nanocavities of Supramolecular Hosts
Authors: Gavvala, Krishna
Sengupta, Abhigyan
HAZRA, PARTHA
Dept. of Chemistry
Keywords: Modulation of Photophysics
pKa Shift
Anti-cancer Drug
Nanocavities
Supramolecular Hosts
State Measurements
2013
Issue Date: Feb-2013
Publisher: Wiley
Citation: ChemPhysChem, 14(3), 532-542.
Abstract: This article reports the pKa shift of an anti‐cancer drug, 20(S)‐camptothecin (CPT), upon encapsulation into the nanocavity of a cucurbit[7]uril (CB7) macrocycle. Steady‐state, time‐resolved fluorescence and electrospray ionisation mass spectrometry (ESI‐MS) studies provide evidence for the formation of both 1:1 and 2:1 (CB7⋅CPT) stoichiometries. Astonishingly, we have found that protonation of CPT takes place at a higher concentration of macrocycle (≥50 μM) when the 2:1 stoichiometric complex develops. However, we did not find any proof for protonation of CPT when it is encased by a β‐cyclodextrin cavity, which has a cavity size almost the same as that of CB7. Hence, we conclude that electron‐rich carbonyl portals of CB7 have an important role in protonation of the drug in the 2:1 inclusion complex. Docking and semi‐empirical quantum chemical calculations have been employed to gain an insight into the molecular picture of orientation of CPT in the inclusion complexes. It is clearly seen from the optimised structure of the 2:1 (CB7⋅CPT) inclusion complex that the quinoline nitrogen of CPT does not reside within either of the CB7 cavities, rather it is almost sandwiched between two CB7 rings, and therefore, it experiences huge electron density exerted by both carbonyl portals of the macrocycles. As a result, the pKa of CPT shifts from 1.2 to 6.2. Finally, controlled release of the drug has been achieved through the introduction of NaCl, which is rich in cells, as an external stimulus. We hope this recognition‐mediated binding and release mechanism can be useful for activation of the drug and controlled release of the drug in therapeutic uses.
URI: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/1591
https://doi.org/10.1002/cphc.201200879
ISSN: 1439-4235
1439-7641
Appears in Collections:JOURNAL ARTICLES

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