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Title: Quantum Dot Nanometal Surface Energy Transfer Based Biosensing of Sialic Acid Compositions and Linkages in Biological Samples
Padler-Karavani, Vered
Diaz, Sandra
Verhagen, Andrea
Yu, Hai
Cao, Hongzhi
Langereis, Martijn A.
Groot, Raoul J. De
Chen, Xi
Varki, Ajit
Dept. of Chemistry
Keywords: Quantum Dot
Nanometal Surface Energy
Sialic Acid Compositions
Linkages in Biological Samples
Nanotechnology-based biosensors
Issue Date: Apr-2013
Publisher: American Chemical Society
Citation: Analytical Chemistry,85(8),
Abstract: Current methods for analyzing sialic acid diversity in modifications and linkages require multistep processing, derivatization, and chromatographic analyses. We here report a single-step optical method for identification and quantification of different compositions of sialoglycans on glycoproteins and in serum. This was achieved by measuring and quantifying nanometal surface energy transfer (NSET) signals between quantum dots and gold nanoparticles bound to specific sialic acid binding proteins (SBPs) and sialic acid moieties, respectively. The biosensing process is based on the NSET turn-on by external sialic acid species that compete for binding to the SBPs. Selectivity of the biosensor toward sialoglycans can be designed to detect the total amount, glycosylation linkages (α2–6 vs α2–3), and modifications (9-O-acetyl and N-glycolyl groups) in the samples. This nanobiosensor is a prototype expected to achieve limits of the detection down to the micromolar range for high-throughput quantification and analysis of different compositions of sialoglycans present in biological or biomedical samples.
ISSN: Mar-00
Appears in Collections:JOURNAL ARTICLES

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