Please use this identifier to cite or link to this item: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/3180
Title: Nup358 binds to AGO proteins through its SUMO-interacting motifs and promotes the association of target mRNA with miRISC
Authors: Sahoo, Manas Ranjan
Gaikwad, Swati
Khuperkar, Deepak
Ashok, Maitreyi
Helen, Mary
Yadav, Santosh Kumar
Singh, Aditi
Magre, Indrasen
Deshmukh, Prachi
Dhanvijay, Supriya
Sahoo, Pabitra Kumar
Ramtirtha, Yogendra
MADHUSUDHAN, M. S.
PANANGHAT, GAYATHRI
Seshadri, Vasudevan
Joseph, Jomon
Dept. of Biology
Keywords: Nup358 binds
AGO proteins
SUMO?interacting motifs
mRNA with miRISC
Annulate Lamellae
Argonaute MiRNA
Nucleoporin
Nup358
2017
Issue Date: Feb-2017
Publisher: EMBO Press
Citation: EMBO Reports, 18, 241-263.
Abstract: MicroRNA (miRNA)‐guided mRNA repression, mediated by the miRNA‐induced silencing complex (miRISC), is an important component of post‐transcriptional gene silencing. However, how miRISC identifies the target mRNA in vivo is not well understood. Here, we show that the nucleoporin Nup358 plays an important role in this process. Nup358 localizes to the nuclear pore complex and to the cytoplasmic annulate lamellae (AL), and these structures dynamically associate with two mRNP granules: processing bodies (P bodies) and stress granules (SGs). Nup358 depletion disrupts P bodies and concomitantly impairs the miRNA pathway. Furthermore, Nup358 interacts with AGO and GW182 proteins and promotes the association of target mRNA with miRISC. A well‐characterized SUMO‐interacting motif (SIM) in Nup358 is sufficient for Nup358 to directly bind to AGO proteins. Moreover, AGO and PIWI proteins interact with SIMs derived from other SUMO‐binding proteins. Our study indicates that Nup358–AGO interaction is important for miRNA‐mediated gene silencing and identifies SIM as a new interacting motif for the AGO family of proteins. The findings also support a model wherein the coupling of miRISC with the target mRNA could occur at AL, specialized domains within the ER, and at the nuclear envelope.
URI: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/3180
https://doi.org/10.15252/embr.201642386
ISSN: 1469-221X
1469-3178
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