Please use this identifier to cite or link to this item: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/3221
Title: Inhibition of copper-mediated aggregation of human γD-crystallin by Schiff bases
Authors: Chauhan, Priyanka
Muralidharan, Sai Brinda
Anand Babu, Velappan
DATTA, DHRUBAJYOTI
Pratihar, Sanjay
Debnath, Joy
Ghosh, Kalyan Sundar
Dept. of Chemistry
Keywords: Inhibition of copper-mediated
Schiff bases
Schiff base
Copper complex
γD-Crystallin
γD-Crystallin
Purification of HGD
2017
Issue Date: Jun-2017
Publisher: Springer Nature
Citation: JBIC Journal of Biological Inorganic Chemistry, 22(4), 505-517.
Abstract: Protein aggregation, due to the imbalance in the concentration of Cu2+ and Zn2+ ions is found to be allied with various physiological disorders. Copper is known to promote the oxidative damage of β/γ-crystallins in aged eye lens and causes their aggregation leading to cataract. Therefore, synthesis of a small-molecule ‘chelator’ for Cu2+ with complementary antioxidant effect will find potential applications against aggregation of β/γ-crystallins. In this paper, we have reported the synthesis of different Schiff bases and studied their Cu2+ complexation ability (using UV–Vis, FT-IR and ESI-MS) and antioxidant activity. Further based on their copper complexation efficiency, Schiff bases were used to inhibit Cu2+-mediated aggregation of recombinant human γD-crystallin (HGD) and β/γ-crystallins (isolated from cataractous human eye lens). Among these synthesized molecules, compound 8 at a concentration of 100 μM had shown ~95% inhibition of copper (100 μM)-induced aggregation. Compound 8 also showed a positive cooperative effect at a concentration of 5–15 μM on the inhibitory activity of human αA-crystallin (HAA) during Cu2+-induced aggregation of HGD. It eventually inhibited the aggregation process by additional ~20%. However, ~50% inhibition of copper-mediated aggregation of β/γ-crystallins (isolated from cataractous human eye lens) was recorded by compound 8 (100 μM). Although the reductive aminated products of the imines showed better antioxidant activity due to their lower copper complexing ability, they were found to be non-effective against Cu2+-mediated aggregation of HGD.
URI: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/3221
https://doi.org/10.1007/s00775-016-1433-0
ISSN: 0949-8257
1432-1327
Appears in Collections:JOURNAL ARTICLES

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.