Please use this identifier to cite or link to this item: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/4460
Title: High-throughput metabolomic and transcriptomic analyses vet the potential route of cerpegin biosynthesis in two varieties of Ceropegia bulbosa Roxb
Authors: Gharat, Sachin A.
Shinde, Balkrishna A.
Mule, Ravindra D.
Punekar, Sachin A.
DHOLAKIA, BHUSHAN B.
Jayaramaiah, Ramesha H.
Ramaswamy, Gopalakrishna
Giri, Ashok P.
Dept. of Biology
Keywords: Metabolite
Cerpegin
Transcriptome
LC-MS
5-Allyl cerpegin
Ceropegia
TOC-FEB-2020
2020
Issue Date: Jan-2020
Publisher: Springer Nature
Citation: Planta, 251(1).
Abstract: Ceropegia bulbosa is an important medicinal plant, used in the treatment of various ailments including diarrhea, dysentery, and syphilis. This is primarily attributed to the presence of pharmaceutically active secondary metabolites, especially cerpegin. As this plant belongs to an endemic threatened category, genomic resources are not available hampering exploration on the molecular basis of cerpegin accumulation till now. Therefore, we undertook high-throughput metabolomic and transcriptomic analyses using different tissues from two varieties namely, C. bulbosa var. bulbosa and C. bulbosa var. lushii. Metabolomic analysis revealed spatial and differential accumulation of various metabolites. We chemically synthesized and characterized the cerpegin and its derivatives by liquid chromatography tandem–mass spectrometry (LC–MS/MS). Importantly, these comparisons suggested the presence of cerpegin and 5-allyl cerpegin in all C. bulbosa tissues. Further, de novo transcriptome analysis indicated the presence of significant transcripts for secondary metabolic pathways through the Kyoto encyclopedia of genes and genomes database. Tissue-specific profiling of transcripts and metabolites showed a significant correlation, suggesting the intricate mechanism of cerpegin biosynthesis. The expression of potential candidate genes from the proposed cerpegin biosynthetic pathway was further validated by qRT-PCR and NanoString nCounter. Overall, our findings propose a potential route of cerpegin biosynthesis. Identified transcripts and metabolites have built a foundation as new molecular resources that could facilitate future research on biosynthesis, regulation, and engineering of cerpegin or other important metabolites in such non-model plants.
URI: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/4460
https://doi.org/10.1007/s00425-019-03319-8
ISSN: 0032-0935
1432-2048
Appears in Collections:JOURNAL ARTICLES

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.