Genetic polymorphism in TLR2 genes of HF cross bred cattle through PCR SSCP

Abstract

TLRs are cell-surface receptors which recognize a broad class of pathogen-associated molecular patterns (PAMPs) that activates innate and adaptive immune responses. Thorough perusal of literature suggests a possible role of TLR2 gene in resistance to infection due to gram positive bacteria. Bovine TLR2 assigns to Bos taurus (BTA) chromosome 17 it spans over 13.2 kb of genomic DNA which includes 2 exons. The first exon is spread over 179bp while the second one is 3333bp. Molecular characterization of TLR2 gene, carried out by PCR- SSCP analysis, revealed presence of six SNPs viz., A827G (exon 2.2), C1088G (exon 2.3), C2155A (exon 2.5), G2281C (exon 2.5), G2410A (exon 2.6) and C2600T (exon 2.6). Gene and genotypic frequencies were determined by values for allele frequencies and hetrozygosity. The degree of heterozygosities, c2 (Chi-square) test for Hardy Weinberg (HW) equilibrium and F statistics were performed for the HF crossbred population. Population genetic analysis demonstrated high degree of genetic differentiation in the population. The studied population displayed a deviation from the HW equilibrium.