Please use this identifier to cite or link to this item:
http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/5695
Title: | Rational designing of glyco-nanovehicles to target cellular heterogeneity |
Authors: | JAIN, PRASHANT SHANTHAMURTHY, CHETHAN D. CHAUDHARY, PREETI MADHUKAR KIKKERI, RAGHAVENDRA Dept. of Chemistry |
Keywords: | Chemistry 2021-MAR-WEEK1 TOC-MAR-2021 2021 |
Issue Date: | Mar-2021 |
Publisher: | Royal Society of Chemistry |
Citation: | Chemical Science, 12(11), 4021-4027. |
Abstract: | The aberrant expression of endocytic epidermal growth factor receptors (EGFRs) in cancer cells has emerged as a key target for therapeutic intervention. Here, we describe for the first time a state-of-the-art design for a heparan sulfate (HS) oligosaccharide-based nanovehicle to target EGFR-overexpressed cancer cells in cellular heterogeneity. An ELISA plate IC50 inhibition assay and surface plasma resonance (SPR) binding assay of structurally well-defined HS oligosaccharides showed that 6-O-sulfation (6-O-S) and 6-O-phosphorylation (6-O-P) of HS tetrasaccharides significantly enhanced EGFR cognate growth factor binding. The conjugation of these HS ligands to multivalent fluorescent gold nanoparticles (AuNPs) enabled the specific and efficient targeting of EGFR-overexpressed cancer cells. In addition, this heparinoid-nanovehicle exhibited selective homing to NPs in cancer cells in three-dimensional (3D) coculture spheroids, thus providing a novel target for cancer therapy and diagnostics in the tumor microenvironment (TME). |
URI: | http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/5695 https://doi.org/10.1039/D1SC00140J |
ISSN: | 2041-6539 |
Appears in Collections: | JOURNAL ARTICLES |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.