Please use this identifier to cite or link to this item: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/5837
Title: Label-free quantitative proteomics of Sorghum bicolor reveals the proteins strengthening plant defense against insect pest Chilo partellus
Authors: Tamhane, Vaijayanti A.
SANT, SURHUD S.
Jadhav, Abhilash R.
War, Abdul R.
Sharma, Hari C.
Jaleel, Abdul
Kashikar, Akanksha S.
Dept. of Biology
Keywords: Chilo partellus
Insect pests
In-solution proteomics
Plant defense
Label-free quantitative proteomics
2021-APR-WEEK3
TOC-APR-2021
2021
Issue Date: Apr-2021
Publisher: Springer Nature
Citation: Proteome Science, 19, 6.
Abstract: Background:Spotted stem borer- Chilo partellus - a Lepidopteran insect pest of Sorghum bicolor is responsible for major economic losses. It is an oligophagous pest, which bores through the plant stem, causing ‘deadheart’ and hampering the development of the main cob. We applied a label-free quantitative proteomics approach on three genotypes of S. bicolor with differential resistance/ susceptibility to insect pests, intending to identify the S. bicolor’s systemic protein complement contributing to C. partellus tolerance. Methods: The proteomes of S. bicolor with variable resistance to insect pests, ICSV700, IS2205 (resistant) and Swarna (susceptible) were investigated and compared using label-free quantitative proteomics to identify putative leaf proteins contributing to resistance to C. partellus. Results:The multivariate analysis on a total of 967 proteins led to the identification of proteins correlating with insect resistance/susceptibility of S. bicolor. Upon C. partellus infestation S. bicolor responded by suppression of protein and amino acid biosynthesis, and induction of proteins involved in maintaining photosynthesis and responding to stresses. The gene ontology analysis revealed that C. partellus-responsive proteins in resistant S. bicolor genotypes were mainly involved in stress and defense, small molecule biosynthesis, amino acid metabolism, catalytic and translation regulation activities. At steady-state, the resistant S. bicolor genotypes displayed at least two-fold higher numbers of unique proteins than the susceptible genotype Swarna, mostly involved in catalytic activities. Gene expression analysis of selected candidates was performed on S. bicolor by artificial induction to mimic C. partellus infestation.
URI: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/5837
https://doi.org/10.1186/s12953-021-00173-z
ISSN: 1477-5956
Appears in Collections:JOURNAL ARTICLES

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