Please use this identifier to cite or link to this item: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/6265
Full metadata record
DC FieldValueLanguage
dc.contributor.advisorRagunath, Vinothkumar Kuttien_US
dc.contributor.authorBORKAR, JANHAVIen_US
dc.date.accessioned2021-09-16T03:48:34Z-
dc.date.available2021-09-16T03:48:34Z-
dc.date.issued2021-09en_US
dc.identifier.citation33en_US
dc.identifier.urihttp://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/6265-
dc.description.abstractN,N-dimethylformamide (DMF) is a widely used organic solvent due to its hydrophilic and highly stable nature. However, it is also a potent pollutant which requires bioremediation solutions. The enzyme Dimethylformamidase (DMFase), extracted from the bacterium Paracoccus sp. strain DMF, can cleave the highly stable amide bond present in DMF. The structure of DMFase was recently published, showing that it is an α2β2 heterotertameric complex with an unusual mononuclear Fe3+ center. A reaction mechanism has been proposed, however, the exact role of the residues present in the active site is not yet known due to the unavailability of the structure of an enzyme-substrate complex. In this project, I studied the role of different residues in the active site using site-directed mutagenesis, isothermal calorimetry and structural analysis. The results from the activity assays of the mutants along with the structure of the E657D mutant provide further support to the proposed mechanism. Mutagenesis studies have also revealed a few other residues that can play a role in the catalytic reaction.en_US
dc.language.isoenen_US
dc.subjectDMFen_US
dc.subjectDimethylformamidaseen_US
dc.subjectDimethylformamideen_US
dc.subjectBiochemistryen_US
dc.subjectEnzymologyen_US
dc.subjectCryo electron microscopyen_US
dc.subjectCryo EMen_US
dc.subjectN,N-dimethylformamideen_US
dc.subjectN,N-Dimethylmethanamideen_US
dc.titleTowards a Mechanism for the Hydrolysis of Dimethylformamide by the Enzyme Dimethylformamidaseen_US
dc.typeThesisen_US
dc.type.degreeBS-MSen_US
dc.contributor.departmentDept. of Biologyen_US
dc.contributor.registration20161172en_US
Appears in Collections:MS THESES

Files in This Item:
File Description SizeFormat 
MSThesis_Janhavi_20161172_Sep15_2021_final_signed.pdf10.09 MBAdobe PDFView/Open    Request a copy


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.