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DC Field | Value | Language |
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dc.contributor.author | MEHENDALE, NEELAY | en_US |
dc.contributor.author | Mallik, Roop | en_US |
dc.contributor.author | KAMAT, SIDDHESH S. | en_US |
dc.date.accessioned | 2021-10-18T10:31:14Z | |
dc.date.available | 2021-10-18T10:31:14Z | |
dc.date.issued | 2021-12 | en_US |
dc.identifier.citation | ACS Chemical Biology, 16(12), 2757–2765. | en_US |
dc.identifier.issn | 1554-8929 | en_US |
dc.identifier.issn | 1554-8937 | en_US |
dc.identifier.uri | http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/6327 | |
dc.identifier.uri | https://doi.org/10.1021/acschembio.1c00393 | en_US |
dc.description.abstract | Phagocytosis is an important physiological process, which, in higher organisms, is a means of fighting infections and clearing cellular debris. During phagocytosis, detrimental foreign particles (e.g. pathogens and apoptotic cells) are engulfed by phagocytes (e.g. macrophages), enclosed in membrane-bound vesicles called phagosomes, and transported to the lysosome for eventual detoxification. During this well-choreographed process, the nascent phagosome (also called early phagosome, EP) undergoes a series of spatiotemporally regulated changes in its protein and lipid composition and matures into a late phagosome (LP), which subsequently fuses with the lysosomal membrane to form the phagolysosome. While several elegant proteomic studies have identified the role of unique proteins during phagosomal maturation, the corresponding lipidomic studies are sparse. Recently, we reported a comparative lipidomic analysis between EPs and LPs and showed that ceramides are enriched on the LPs. Further, we found that this ceramide accumulation on LPs was orchestrated by ceramide synthase 2, inhibition of which hampers phagosomal maturation. Following up on this study, here, using biochemical assays, we first show that the increased ceramidase activity on EPs also significantly contributes to the accumulation of ceramides on LPs. Next, leveraging lipidomics, we show that de novo ceramide synthesis does not significantly contribute to the ceramide accumulation on LPs, while concomitant to increased ceramides, glucosylceramides are substantially elevated on LPs. We validate this interesting finding using biochemical assays and show that LPs indeed have heightened glucosylceramide synthase activity. Taken together, our studies provide interesting insights and possible new roles of sphingolipid metabolism during phagosomal maturation. | en_US |
dc.language.iso | en | en_US |
dc.publisher | American Chemical Society | en_US |
dc.subject | Biology | en_US |
dc.subject | 2021-OCT-WEEK1 | en_US |
dc.subject | TOC-OCT-2021 | en_US |
dc.subject | 2021 | en_US |
dc.title | Mapping Sphingolipid Metabolism Pathways during Phagosomal Maturation | en_US |
dc.type | Article | en_US |
dc.contributor.department | Dept. of Biology | en_US |
dc.identifier.sourcetitle | ACS Chemical Biology | en_US |
dc.publication.originofpublisher | Foreign | en_US |
Appears in Collections: | JOURNAL ARTICLES |
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