Please use this identifier to cite or link to this item: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/6327
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dc.contributor.authorMEHENDALE, NEELAYen_US
dc.contributor.authorMallik, Roopen_US
dc.contributor.authorKAMAT, SIDDHESH S.en_US
dc.date.accessioned2021-10-18T10:31:14Z
dc.date.available2021-10-18T10:31:14Z
dc.date.issued2021-12en_US
dc.identifier.citationACS Chemical Biology, 16(12), 2757–2765.en_US
dc.identifier.issn1554-8929en_US
dc.identifier.issn1554-8937en_US
dc.identifier.urihttp://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/6327
dc.identifier.urihttps://doi.org/10.1021/acschembio.1c00393en_US
dc.description.abstractPhagocytosis is an important physiological process, which, in higher organisms, is a means of fighting infections and clearing cellular debris. During phagocytosis, detrimental foreign particles (e.g. pathogens and apoptotic cells) are engulfed by phagocytes (e.g. macrophages), enclosed in membrane-bound vesicles called phagosomes, and transported to the lysosome for eventual detoxification. During this well-choreographed process, the nascent phagosome (also called early phagosome, EP) undergoes a series of spatiotemporally regulated changes in its protein and lipid composition and matures into a late phagosome (LP), which subsequently fuses with the lysosomal membrane to form the phagolysosome. While several elegant proteomic studies have identified the role of unique proteins during phagosomal maturation, the corresponding lipidomic studies are sparse. Recently, we reported a comparative lipidomic analysis between EPs and LPs and showed that ceramides are enriched on the LPs. Further, we found that this ceramide accumulation on LPs was orchestrated by ceramide synthase 2, inhibition of which hampers phagosomal maturation. Following up on this study, here, using biochemical assays, we first show that the increased ceramidase activity on EPs also significantly contributes to the accumulation of ceramides on LPs. Next, leveraging lipidomics, we show that de novo ceramide synthesis does not significantly contribute to the ceramide accumulation on LPs, while concomitant to increased ceramides, glucosylceramides are substantially elevated on LPs. We validate this interesting finding using biochemical assays and show that LPs indeed have heightened glucosylceramide synthase activity. Taken together, our studies provide interesting insights and possible new roles of sphingolipid metabolism during phagosomal maturation.en_US
dc.language.isoenen_US
dc.publisherAmerican Chemical Societyen_US
dc.subjectBiologyen_US
dc.subject2021-OCT-WEEK1en_US
dc.subjectTOC-OCT-2021en_US
dc.subject2021en_US
dc.titleMapping Sphingolipid Metabolism Pathways during Phagosomal Maturationen_US
dc.typeArticleen_US
dc.contributor.departmentDept. of Biologyen_US
dc.identifier.sourcetitleACS Chemical Biologyen_US
dc.publication.originofpublisherForeignen_US
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