Please use this identifier to cite or link to this item: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/7027
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dc.contributor.authorSAHARE, MAHESH G.en_US
dc.contributor.authorSuyatnoen_US
dc.contributor.authorImai, Hiroshien_US
dc.date.accessioned2022-06-13T04:29:00Z-
dc.date.available2022-06-13T04:29:00Z-
dc.date.issued2018-04en_US
dc.identifier.citationReproductive Medicine and Biology, 17(2), 134-42.en_US
dc.identifier.issn1447-0578en_US
dc.identifier.urihttps://doi.org/10.1002/rmb2.12087en_US
dc.identifier.urihttp://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/7027-
dc.description.abstractBackground:Spermatogonial stem cells (SSCs) in the mammalian testis are unipotent stem cells for spermatozoa. They show unique cell characteristics as stem cells and germ cells after being isolated from the testis and cultured in vitro. This review introduces recent progress in the development of culture ystems for the establishment of SSC lines in mammalian species, including humans. Methods:Based on the published reports, the isolation and purification of SSCs, identification and characteristics of SSCs, and culture system for mice, humans, and domestic animals have been summarized. Results:In mice, cell lines from SSCs are established and can be reprogrammed to show pluripotent stem cell potency that is similar to embryonic stem cells. However, it is difficult to stablish cell lines for animals other than mice because of the dearth of understanding about species-specific requirements for growth factors and mechanisms upporting the self-renewal of cultured SSCs. Among the factors that are associated with the development of culture systems, the enrichment of SSCs that are isolated from the testis and the combination of growth factors are essential. Conclusion:Providing an example of SSC culture in cattle, a rational consideration was made about how it can be possible to establish cell lines from neonatal and immature testes.en_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.subjectBiologyen_US
dc.subject2018en_US
dc.titleRecent advances of in vitro culture systems for spermatogonial stem cells in mammalsen_US
dc.typeArticleen_US
dc.contributor.departmentDept. of Biologyen_US
dc.identifier.sourcetitleReproductive Medicine and Biologyen_US
dc.publication.originofpublisherForeignen_US
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