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dc.contributor.authorShintre, Neha A.en_US
dc.contributor.authorTamhane, Vaijayanti A.en_US
dc.contributor.authorBAIG, ULFAT I.en_US
dc.contributor.authorPund, Anagha S.en_US
dc.contributor.authorPatwardhan, Rajashree B.en_US
dc.contributor.authorDeshpande, Neelima M.en_US
dc.date.accessioned2022-06-13T04:29:21Z-
dc.date.available2022-06-13T04:29:21Z-
dc.date.issued2020-09en_US
dc.identifier.citationCurrent Microbiology, 77, 3555–3564.en_US
dc.identifier.issn1432-0991en_US
dc.identifier.issn0343-8651en_US
dc.identifier.urihttps://doi.org/10.1007/s00284-020-02174-1en_US
dc.identifier.urihttp://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/7050-
dc.description.abstractPhylogenetic diversity of culturable actinobacteria isolated from the intertidal regions of west coast of Maharashtra, India was studied using 16S rRNA gene sequencing. Total of 140 actinobacterial isolates were obtained, which belonged to 14 genera, 10 families and 65 putative species with Streptomyces being the most dominant (63%) genus followed by Nocardiopsis and Micromonospora. Isolates were screened for production of extracellular protease inhibitors (PI) against three pure proteases viz. chymotrypsin, trypsin, subtilisin and a crude extracellular protease from Pseudomonas aeruginosa. Eighty percent of the isolates showed PI activity against at least one of the four proteases, majority of these belonged to genus Streptomyces. Actinobacterial diversity from two sites Ade (17° 52′ N, 73° 04′ E) and Harnai (17° 48′ N, 73° 05′ E) with varying anthropological pressure showed that more putative species diversity was obtained from site with lower human intervention i.e. Ade (Shannon’s H 3.45) than from Harnai (Shannon’s H 2.83), a site with more human intervention. However, in Ade, percentage of isolates not showing PI activity against any of the proteases was close to 21% and that in Harnai was close to 9%. In other words, percentage of PI producers was lower at a site with lesser human intervention.en_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.subjectBiologyen_US
dc.subject2020en_US
dc.titleDiversity of Culturable Actinobacteria Producing Protease Inhibitors Isolated from the Intertidal Zones of Maharashtra, Indiaen_US
dc.typeArticleen_US
dc.contributor.departmentDept. of Biologyen_US
dc.identifier.sourcetitleCurrent Microbiologyen_US
dc.publication.originofpublisherForeignen_US
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