Inducible CRISPR/CAS9 genome editing to modulate autophagy during de novo organ regeneration

Abstract

Regeneration is a natural process that gets activated upon injury and helps reorganize the lost body parts so that it regains their full functionality. At the cellular level, one of many intriguing questions to ask is how the cell fixes its integrity during an extensive damage condition and how it forms an organ de novo. Autophagy is identified as one of the cell survival mechanisms that help maintain homeostasis in damaged cells by the elimination of damaged organelles. However, little is known about the pathways that could link autophagy and regeneration in the plant kingdom. Therefore, this study on creating knockouts of autophagy genes with Inducible CRISPR/Cas9 Genome Editing would potentially throw light on the molecular and genetic regulation of regeneration via autophagy in wound-induced de novo organogenesis.