Please use this identifier to cite or link to this item: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/7939
Title: A Role for the Orphan Serine hydrolase CG17192 in the Drosophila immune response
Authors: RATNAPARKHI, GIRISH
PAZARE, MRUNAL
Dept. of Biology
20181184
Keywords: Research Subject Categories:: Drosophila
Research Subject Categories:: Serine Hydrolase
Research Subject Categories::Immunity
Issue Date: May-2023
Citation: 58
Abstract: Highly conserved during evolution, the Serine Hydrolase (SH) superfamily of enzymes is a diverse group of catalysts that conduct a wide range of metabolic events in eukaryotic cells. A recent characterization of the activation of SHs in Drosophila (Kumar et al. 2021) found that the enzymatic activity of CG17192 is enriched in the female hemolymph and male gut. CG17192 is anticipated to be involved in degrading and absorbing a significant fraction (~ 30%) of adult triglycerides in the animal diet. As part of my MS thesis project, I have initiated the functional characterization of CG17192 using in vitro biochemical assays, activity-based proteomics, and lipidomics. I have validated the activity of the SH in-vitro using ABPP (Activity-based protein pro- filing) assay, utilizing the broad-spectrum chemical probe flurophosphonate tagged with either rhodamine. For in-vitro experiments, I have cloned CG17192 for expression in both bacteria and insect cells. Additionally, I have generated a catalytically dead CG17192S179A mutant. Loss of function studies in the midgut via RNAi indicate that CG17192 may function as an immunosuppressor.
URI: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/7939
Appears in Collections:MS THESES

Files in This Item:
File Description SizeFormat 
20181184_Mrunal_Ashok_Pazare_MS_ThesisMS Thesis3.98 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.