Please use this identifier to cite or link to this item: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/8062
Title: Microscopic Mechanism of Macromolecular Crowder-Assisted DNA Capture and Translocation through Biological Nanopores
Authors: PUNIA, BHAWAKSHI
CHAUDHURY, SRABANTI
Dept. of Chemistry
Keywords: Genetics
Kinetic parameters
Mathematical methods
Molecules
Nanopores
2023-JUN-WEEK4
TOC-JUN-2023
2023
Issue Date: Jul-2023
Publisher: American Chemical Society
Citation: Journal of Physical Chemistry B
Abstract: Biological nanopore sensors are widely used for genetic sequencing as nucleic acids and other molecules translocate through them across membranes. Recent studies have shown that the transport of these polymers through nanopores is strongly influenced by macromolecular bulk crowders. By using poly(ethylene glycol) (PEG) molecules as crowders, experiments have shown an increase in the capture rates and translocation times of polymers through an α-hemolysin (αHL) nanopore, which provides high-throughput signals and accurate sensing. A clear molecular-level understanding of how the presence of PEGs offers such desirable outcomes in nanopore sensing is still missing. In this work, we present a new theoretical approach to probe the effect of PEG crowders on DNA capture and translocation through the αHL nanopore. We develop an exactly solvable discrete-state stochastic model based on the cooperative partitioning of individual polycationic PEGs within the cavity of the αHL nanopore. It is argued that the apparent electrostatic interactions between the DNA and PEGs control all of the dynamic processes. Our analytical predictions find excellent agreements with existing experiments, thereby strongly supporting our theory.
URI: https://doi.org/10.1021/acs.jpcb.3c02792
http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/8062
ISSN: 1520-6106
1520-5207
Appears in Collections:JOURNAL ARTICLES

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