Please use this identifier to cite or link to this item: http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/9147
Title: Immunogenic Sulfated l-Idose Homo Oligosaccharides Elicit Neutralizing Antibody against Native Heparan Sulfate with Biomarker and Therapeutic Possibilities
Authors: VISHWESHWARA, SHARATH S.
BHOGE, PREETI RAVINDRA
ANAND, SAURABH
RAIGAWALI, RAKESH
CHANDRA, ANKITA
Saladi, Srinivas Vinod
KIKKERI, RAGHAVENDRA
Dept. of Chemistry
Keywords: Biopolymers
Immunology
Ligands
Mixtures
Peptides and proteins
2024
2024-OCT-WEEK2
TOC-OCT-2024 
Issue Date: Oct-2024
Publisher: American Chemical Society
Citation: Journal of Medicinal Chemistry, 67(20), 18465–18477.
Abstract: Heparan sulfate (HS) is a non-immunogenic antigen, and developing antibodies against specific sulfated patterns in HS poses significant challenges. Herein, we employed an innovative immunization strategy that exploits the molecular mimicry of HS to generate antibodies against HS sequences. Mice were immunized with synthetic sulfated oligo-l-idose (ID49) that mimics optimum 67% of the conserved structure of HS ligands. This immunization of ID49@CRM197 with alum and Freund’s adjuvant resulted in the production of robust IgG antibody responses targeting ID49 and cross-reactivity with the N-sulfated HS ligands compared to N-unsubstituted and N-acetate domain synthetic HS ligands. Such a pharmacological agent exhibited distinct staining of tissue sections and cell lines and induced complement-dependent cell cytotoxicity against SK-BR-3 cancer cells. Moreover, these antibodies inhibited heparin-mediated anticoagulation activity similar to that of protamine. These findings highlight the biomarker and possible therapeutic capability of the antibodies.
URI: https://doi.org/10.1021/acs.jmedchem.4c01772
http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/9147
ISSN: 0022-2623
1520-4804
Appears in Collections:JOURNAL ARTICLES

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