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dc.contributor.authorVISHWESHWARA, SHARATH S.en_US
dc.contributor.authorBHOGE, PREETI RAVINDRAen_US
dc.contributor.authorANAND, SAURABHen_US
dc.contributor.authorRAIGAWALI, RAKESHen_US
dc.contributor.authorCHANDRA, ANKITAen_US
dc.contributor.authorSaladi, Srinivas Vinoden_US
dc.contributor.authorKIKKERI, RAGHAVENDRAen_US
dc.date.accessioned2024-10-29T06:44:40Z
dc.date.available2024-10-29T06:44:40Z
dc.date.issued2024-10en_US
dc.identifier.citationJournal of Medicinal Chemistry, 67(20), 18465–18477.en_US
dc.identifier.issn0022-2623en_US
dc.identifier.issn1520-4804en_US
dc.identifier.urihttps://doi.org/10.1021/acs.jmedchem.4c01772en_US
dc.identifier.urihttp://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/9147
dc.description.abstractHeparan sulfate (HS) is a non-immunogenic antigen, and developing antibodies against specific sulfated patterns in HS poses significant challenges. Herein, we employed an innovative immunization strategy that exploits the molecular mimicry of HS to generate antibodies against HS sequences. Mice were immunized with synthetic sulfated oligo-l-idose (ID49) that mimics optimum 67% of the conserved structure of HS ligands. This immunization of ID49@CRM197 with alum and Freund’s adjuvant resulted in the production of robust IgG antibody responses targeting ID49 and cross-reactivity with the N-sulfated HS ligands compared to N-unsubstituted and N-acetate domain synthetic HS ligands. Such a pharmacological agent exhibited distinct staining of tissue sections and cell lines and induced complement-dependent cell cytotoxicity against SK-BR-3 cancer cells. Moreover, these antibodies inhibited heparin-mediated anticoagulation activity similar to that of protamine. These findings highlight the biomarker and possible therapeutic capability of the antibodies.en_US
dc.language.isoenen_US
dc.publisherAmerican Chemical Societyen_US
dc.subjectBiopolymersen_US
dc.subjectImmunologyen_US
dc.subjectLigandsen_US
dc.subjectMixturesen_US
dc.subjectPeptides and proteinsen_US
dc.subject2024en_US
dc.subject2024-OCT-WEEK2en_US
dc.subjectTOC-OCT-2024 en_US
dc.titleImmunogenic Sulfated l-Idose Homo Oligosaccharides Elicit Neutralizing Antibody against Native Heparan Sulfate with Biomarker and Therapeutic Possibilitiesen_US
dc.typeArticleen_US
dc.contributor.departmentDept. of Chemistryen_US
dc.identifier.sourcetitleJournal of Medicinal Chemistryen_US
dc.publication.originofpublisherForeignen_US
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