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Evolutionary mechanisMS of asymmetric spindle positioning in nematode embyos

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dc.contributor.advisor ATHALE, CHAITANYA A. en_US
dc.contributor.author SETHI, TANMAYA en_US
dc.date.accessioned 2018-05-17T03:21:04Z
dc.date.available 2018-05-17T03:21:04Z
dc.date.issued 2018-05 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/1007
dc.description.abstract Asymmetric cell division is essential for generating cellular diversity, which leads to two unequal daughter cells with different cellular fates. In most of the animal cells, asymmetric spindle positioning is the key factor which leads to the first asymmetric cell division and decides the size of the daughter cells. Although the process of asymmetric positioning of spindle is conserved in most of the species, but not much is known about the robustness of the mechanisms which lead to this evolutionarily conserved phenomenon. We recorded the first embryonic division three nematode species such as Caenorhabditis elegans, Oscheius tipulae and Diploscapter sp.JU359 to study the biophysical properties of spindle positioning and the mechanical parameters those can affect the spindle positioning. We hypothesized that viscosity could be one of the parameters which might affect the different mechanisms which govern the asymmetric spindle positioning. So, by using microrheology methods we tried calculating viscosity values of the cytoplasm of these nematode species by tracking the lipid granules in the cytoplasm. The results showed us that, Diploscapter sp.JU359 which has the least movement of spindle has the highest viscosity values and all the three species show higher viscosity values than water. en_US
dc.description.sponsorship Charpak Exchange scholarship, CNRS, DST Inspire fellowship en_US
dc.language.iso en en_US
dc.subject 2018
dc.subject Biology en_US
dc.subject Asymmetric spindle positioning en_US
dc.subject Oscillations in nematodes en_US
dc.title Evolutionary mechanisMS of asymmetric spindle positioning in nematode embyos en_US
dc.type Thesis en_US
dc.type.degree BS-MS en_US
dc.contributor.department Dept. of Biology en_US
dc.contributor.registration 20131132 en_US


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  • MS THESES [1705]
    Thesis submitted to IISER Pune in partial fulfilment of the requirements for the BS-MS Dual Degree Programme/MSc. Programme/MS-Exit Programme

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