dc.contributor.author |
Sabale, Pramod M. |
en_US |
dc.contributor.author |
Tanpure, Arun A. |
en_US |
dc.contributor.author |
SRIVATSAN, SEERGAZHI G. |
en_US |
dc.date.accessioned |
2018-06-28T04:05:28Z |
|
dc.date.available |
2018-06-28T04:05:28Z |
|
dc.date.issued |
2018-05 |
en_US |
dc.identifier.citation |
Organic & Biomolecular Chemistry. Vol. 16(22). |
en_US |
dc.identifier.issn |
1477-0539 |
en_US |
dc.identifier.uri |
http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/1070 |
|
dc.identifier.uri |
https://doi.org/10.1039/C8OB00646F |
en_US |
dc.description.abstract |
Double-stranded segments of a genome that can potentially form G-quadruplex (GQ) and/or i-motif (iM) structures are considered to be important regulatory elements. Hence, the development of a common probe that can detect GQ and iM structures and also distinguish them from a duplex structure will be highly useful in understanding the propensity of such segments to adopt duplex or non-canonical four-stranded structures. Here, we describe the utility of a conformation-sensitive fluorescent nucleoside analog, which was originally developed as a GQ sensor, in detecting the iM structures of C-rich DNA oligonucleotides (ONs). The analog is based on a 5-(benzofuran-2-yl)uracil scaffold, which when incorporated into C-rich ONs (e.g., telomeric repeats) fluorescently distinguishes an iM from random coil and duplex structures. Steady-state and time-resolved fluorescence techniques enabled the determination of transition pH for the transformation of a random coil to an iM structure. Furthermore, a qualitative understanding on the relative population of duplex and GQ/iM forms under physiological conditions could be gained by correlating the fluorescence, CD and thermal melting data. Taken together, this sensor could provide a general platform to profile double-stranded promoter regions in terms of their ability to adopt four-stranded structures, and also could support approaches to discover functional GQ and iM binders. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Royal Society of Chemistry |
en_US |
dc.subject |
Chemistry |
en_US |
dc.subject |
Common probe |
en_US |
dc.subject |
G-quadruplex |
en_US |
dc.subject |
C-rich DNA oligonucleotides |
en_US |
dc.subject |
TOC-JUNE-2018 |
en_US |
dc.subject |
2018 |
en_US |
dc.title |
Probing the competition between duplex and G-quadruplex/i-motif structures using a conformation-sensitive fluorescent nucleoside probe |
en_US |
dc.type |
Article |
en_US |
dc.contributor.department |
Dept. of Chemistry |
en_US |
dc.identifier.sourcetitle |
Organic & Biomolecular Chemistry |
en_US |
dc.publication.originofpublisher |
Foreign |
en_US |