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RNA interference based Screen to Identify Genetic Modifiers of dVAPB

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dc.contributor.advisor RATNAPARKHI, GIRISH S. en_US
dc.contributor.author VERMA, HEMANT KUMAR en_US
dc.date.accessioned 2011-05-09T05:50:51Z
dc.date.available 2011-05-09T05:50:51Z
dc.date.issued 2011-05 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/129
dc.description.abstract Amyotrophic Lateral Sclerosis (ALS) is a progressive neuro-degenerative disease affecting motor neurons. VAPB- VAMP (vesicle associated membrane protein-associated protein) –associated protein B has been identified as gene involved in type 8 ALS. However, the exact mechanism by which mutation in VAPB causes neuro-degeneration is unknown. Here we present an RNAi based genetic screen to dissect out an integrated genetic network of VAPB function modifiers. We have screened 10% of Drosophila melanogaster genome and have identified 78 modifiers (27 enhancer and 51 suppressors). Modifiers detected in our screen include known ALS causing genes such as SOD1, Alsin2 and TDP-43. Modifiers also include genes that have been implicated in other neurodegenerative diseases such as Parkinson’s disease and Huntington’s disease. We have also identified proteins such as SNAMA, with a known role in apoptosis, and Derlin-1, a component of ER associated degrading machinery as VAPB modifiers. en_US
dc.description.sponsorship IISER PUNE en_US
dc.language.iso en en_US
dc.subject 2011
dc.subject RNAI screen en_US
dc.subject dVAPB en_US
dc.title RNA interference based Screen to Identify Genetic Modifiers of dVAPB en_US
dc.type Thesis en_US
dc.type.degree BS-MS en_US
dc.contributor.department Dept. of Biology en_US
dc.contributor.registration 20061034 en_US


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  • MS THESES [1705]
    Thesis submitted to IISER Pune in partial fulfilment of the requirements for the BS-MS Dual Degree Programme/MSc. Programme/MS-Exit Programme

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