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Fluorescent Nucleoside Analogs: Probes for Investigating Nucleic Acid Structure and Function

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dc.contributor.author Tanpure, Arun A. en_US
dc.contributor.author Pawar, Maroti G. en_US
dc.contributor.author SRIVATSAN, SEERGAZHI G. en_US
dc.date.accessioned 2019-02-14T05:00:43Z
dc.date.available 2019-02-14T05:00:43Z
dc.date.issued 2013-06 en_US
dc.identifier.citation Israel Journal of Chemistry, 53(6-7), en_US
dc.identifier.issn 0021-2148 en_US
dc.identifier.issn 1869-5868 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/1612
dc.identifier.uri https://doi.org/10.1002/ijch.201300010 en_US
dc.description.abstract Base‐modified fluorescent nucleoside analog probes have been very valuable in the study of nucleic acid structure and function. Many of them structurally resemble natural bases, and also display useful properties, such as large Stokes shifts and sensitivity to microenvironment changes. Therefore, unlike traditional fluorescence probes, which mostly report global changes, nucleoside analogs, when incorporated into oligonucleotides, can photophysically report changes that occur around the site of interest, at the nucleotide level. In this review, we provide an overview of various strategies that have been employed to design base‐modified fluorescent nucleoside analogs. Then we review recent developments and applications of new generation fluorescent nucleoside analogs with a particular focus on the synthesis, photophysical characterizations and applications of heterobicycle‐conjugated pyrimidine nucleoside analogs that have been developed by our group. These analogs, which have a minimal effect on the structures of the oligonucleotides into which they are incorporated, show emission in the visible region and excellent fluorescence solvatochromism. Notably, unlike the majority of fluorescent nucleoside analogs developed so far, these analogs retain their fluorescence efficiency when incorporated into oligonucleotides. We anticipate that these nucleoside analogs, with such photophysical properties, would be useful in designing robust biophysical assays to study nucleic acids. en_US
dc.language.iso en en_US
dc.publisher Wiley en_US
dc.subject Fluorescent Nucleoside Analogs en_US
dc.subject Vital cellular functions en_US
dc.subject -Photophysical Properties en_US
dc.subject 2013 en_US
dc.title Fluorescent Nucleoside Analogs: Probes for Investigating Nucleic Acid Structure and Function en_US
dc.type Article en_US
dc.contributor.department Dept. of Chemistry en_US
dc.identifier.sourcetitle Israel Journal of Chemistry en_US
dc.publication.originofpublisher Foreign en_US


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