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Developing Magnetic Tweezers for constant load experiments on microtubule motor proteins

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dc.contributor.advisor Mallik, Roop en_US
dc.contributor.author VENKAT, SWATI en_US
dc.date.accessioned 2012-05-07T06:56:10Z
dc.date.available 2012-05-07T06:56:10Z
dc.date.issued 2012-05 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/193
dc.description.abstract This work reports the construction of magnetic tweezers that can apply a spatially uniform magnetic force on superparamagnetic beads viewed in an optical microscope. The apparatus, constructed and assembled on the current microscope setup is composed of 2 electromagnets and can exert a constant force along an axis perpendicular to the generated field. The instrument was calibrated by measuring the velocity of single 2.8μm dynal magnetic beads near the surface since magnetic beads eventually sink to the surface due to their high density (1.6gm/cc). The maximum magnetic force on the beads, determined using Stoke’s law was found to be 7.29pN at 1.6A current with a spatial variation of 4%. However, since beads near the surface experience higher drag force than the value determined using Stoke’s law, the magnetic force on these beads is higher than estimated. Further work aims to calibrate neutrally buoyant beads suspended in a 3.9M Cesium Chloride solution of equal density (1.6gm/cc). The calibrated instrument will then be used to investigate the nature of single and multiple dynein-driven magnetic phagosome motion in response to constant load. en_US
dc.language.iso en en_US
dc.subject 2012
dc.subject Instrumentation Project en_US
dc.title Developing Magnetic Tweezers for constant load experiments on microtubule motor proteins en_US
dc.type Thesis en_US
dc.type.degree BS-MS en_US
dc.contributor.department Dept. of Biology en_US
dc.contributor.registration 20071045 en_US


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  • MS THESES [1705]
    Thesis submitted to IISER Pune in partial fulfilment of the requirements for the BS-MS Dual Degree Programme/MSc. Programme/MS-Exit Programme

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