dc.contributor.author |
GHOSH, CHANDRAMOULI |
en_US |
dc.contributor.author |
NANDI, ADITI |
en_US |
dc.contributor.author |
Basu, Sudipta |
en_US |
dc.date.accessioned |
2019-03-26T10:01:04Z |
|
dc.date.available |
2019-03-26T10:01:04Z |
|
dc.date.issued |
2019-01 |
en_US |
dc.identifier.citation |
Nanoscale, 11(7), 3326-3335. |
en_US |
dc.identifier.issn |
2040-3364 |
en_US |
dc.identifier.issn |
2040-3372 |
en_US |
dc.identifier.uri |
http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/2386 |
|
dc.identifier.uri |
https://doi.org/10.1039/c8nr08682f |
en_US |
dc.description.abstract |
The endoplasmic reticulum (ER) is one of the most important organelles controlling myriads of cellular functions including protein folding/misfolding/unfolding, calcium ion homeostasis and lipid biosynthesis. Subsequently, due to its functional dysregulation in cancer cells, it has emerged as an interesting target for anti-cancer therapy. However, specific targeting of the ER in cancer cells remains a major challenge due to the lack of ER-selective chemical tools. Furthermore, for performing multiple cellular functions the ER is dependent on the nucleus through complicated cross-talk. Herein, we have engineered a supramolecular self-assembled hexameric rosette structure from two small molecules: tri-substituted triazine and 5-fluorouracil (5-FU). This rosette structure consists of an ER-targeting moiety with a fluorescence tag, an ER-stress inducer and a nuclear DNA damaging drug simultaneously, which further self-assembled into an ER-targeting spherical nano-scale particle (ER-NP). These ER-NPs internalized into HeLa cervical cancer cells by macropinocytosis and specifically localized into the ER to induce ER stress and DNA damage leading to cell death through apoptosis. Interestingly, ER-NPs initiated autophagy, inhibited by a combination of ER-NPs and chloroquine (CQ) to augment cancer cell death. This work has the potential to exploit the concept of supramolecular self-assembly into developing novel nano-scale materials for specific sub-cellular targeting of multiple organelles for future anti-cancer therapy. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Royal Society of Chemistry |
en_US |
dc.subject |
Nanoparticle |
en_US |
dc.subject |
Melamine |
en_US |
dc.subject |
DNA |
en_US |
dc.subject |
Mitochondria |
en_US |
dc.subject |
Peptide |
en_US |
dc.subject |
Trafficking |
en_US |
dc.subject |
Chemistry |
en_US |
dc.subject |
Apoptosis |
en_US |
dc.subject |
Autophagy |
en_US |
dc.subject |
Efficacy |
en_US |
dc.subject |
TOC-MAR-2019 |
en_US |
dc.subject |
2019 |
en_US |
dc.title |
Supramolecular self-assembly of triazine-based small molecules: targeting the endoplasmic reticulum in cancer cells |
en_US |
dc.type |
Article |
en_US |
dc.contributor.department |
Dept. of Chemistry |
en_US |
dc.identifier.sourcetitle |
Nanoscale |
en_US |
dc.publication.originofpublisher |
Foreign |
en_US |