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Dual Functional Nanocarrier for Cellular Imaging and Drug Delivery in Cancer Cells Based on π-Conjugated Core and Biodegradable Polymer Arms

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dc.contributor.author Kulkarni, Bhagyashree en_US
dc.contributor.author Surnar, Bapurao en_US
dc.contributor.author JAYAKANNAN, MANICKAM en_US
dc.date.accessioned 2019-04-29T10:15:49Z
dc.date.available 2019-04-29T10:15:49Z
dc.date.issued 2016-03 en_US
dc.identifier.citation Biomacromolecules, 17 (3), 1004-1016. en_US
dc.identifier.issn 1525-7797 en_US
dc.identifier.issn 1526-4602 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/2711
dc.identifier.uri https://doi.org/10.1021/acs.biomac.5b01654 en_US
dc.description.abstract Multipurpose polymer nanoscaffolds for cellular imaging and delivery of anticancer drug are urgently required for the cancer therapy. The present investigation reports a new polymer drug delivery concept based on biodegradable polycaprolactone (PCL) and highly luminescent π-conjugated fluorophore as dual functional nanocarrier for cellular imaging and delivery vehicles for anticancer drug to cancer cells. To accomplish this goal, a new substituted caprolactone monomer was designed, and it was subjected to ring opening polymerization using a blue luminescent bishydroxyloligo-phenylenevinylene (OPV) fluorophore as an initiator. A series of A–B–A triblock copolymer building blocks with a fixed OPV π-core and variable chain biodegradable PCL arm length were tailor-made. These triblocks self-assembled in organic solvents to produce well-defined helical nanofibers, whereas in water they produced spherical nanoparticles (size ∼150 nm) with blue luminescence. The hydrophobic pocket of the polymer nanoparticle was found to be an efficient host for loading water insoluble anticancer drug such as doxorubicin (DOX). The photophysical studies revealed that there was no cross-talking between the OPV and DOX chromophores, and their optical purity was retained in the nanoparticle assembly for cellular imaging. In vitro studies revealed that the biodegradable PCL arm was susceptible to enzymatic cleavage at the intracellular lysosomal esterase under physiological conditions to release the loaded drugs. The nascent nanoparticles were found to be nontoxic to cancer cells, whereas the DOX-loaded nanoparticles accomplished more than 80% killing in HeLa cells. Confocal microscopic analysis confirmed the cell penetrating ability of the blue luminescent polymer nanoparticles and their accumulation preferably in the cytoplasm. The DOX loaded red luminescent polymer nanoparticles were also taken up by the cells, and the drug was found to be accumulated at the perinuclear environment. The new nanocarrier approach reported in the present manuscript accomplishes both cellular imaging and delivering drugs to intracellular compartments in a single polymer system. The present investigation is one of the first examples to demonstrate the dual functional biodegradable luminescence nanocarrier concept in the literature, and the studies established this proof-of-concept in cellular imaging and drug delivery in cancer cells. en_US
dc.language.iso en en_US
dc.publisher American Chemical Society en_US
dc.subject Dual Functional Nanocarrier en_US
dc.subject Cellular Imaging en_US
dc.subject Drug Delivery en_US
dc.subject Cancer Cells en_US
dc.subject ?-Conjugated Core en_US
dc.subject Biodegradable Polymer Arms en_US
dc.subject 2016 en_US
dc.title Dual Functional Nanocarrier for Cellular Imaging and Drug Delivery in Cancer Cells Based on π-Conjugated Core and Biodegradable Polymer Arms en_US
dc.type Article en_US
dc.contributor.department Dept. of Chemistry en_US
dc.identifier.sourcetitle Biomacromolecules en_US
dc.publication.originofpublisher Foreign en_US


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