dc.contributor.author |
RAO, HARITA |
en_US |
dc.contributor.author |
TANPURE, ARUN A. |
en_US |
dc.contributor.author |
Sawant, Anupam A. |
en_US |
dc.contributor.author |
SRIVATSAN, SEERGAZHI G. |
en_US |
dc.date.accessioned |
2019-07-23T11:08:49Z |
|
dc.date.available |
2019-07-23T11:08:49Z |
|
dc.date.issued |
2012-05 |
en_US |
dc.identifier.citation |
Nature Protocols, 7,1097-1112. |
en_US |
dc.identifier.issn |
1750-2799 |
en_US |
dc.identifier.uri |
http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/3623 |
|
dc.identifier.uri |
https://doi.org/10.1038/nprot.2012.046 |
en_US |
dc.description.abstract |
This protocol describes the detailed experimental procedure for the synthesis of an azide-modified uridine triphosphate analog and its effective incorporation into an oligoribonucleotide by in vitro transcription reactions. Furthermore, procedures for labeling azide-modified oligoribonucleotides post-transcriptionally with biophysical probes by copper(I)-catalyzed alkyne-azide cycloaddition (CuAAC) and Staudinger reactions are also provided. This post-transcriptional chemical modification protocol is simple and modular, and it affords labeled oligonucleotides in reasonable amounts for biophysical assays. The procedure for enzymatic incorporation of the monophosphate of azide-modified UTP into an oligoribonucleotide transcript takes ∼2 d, and subsequent post-transcriptional chemical functionalization of the transcript takes about 2 d. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Nature Publishing Group |
en_US |
dc.subject |
Chemistry |
en_US |
dc.subject |
2012 |
en_US |
dc.title |
Enzymatic incorporation of an azide-modified UTP analog into oligoribonucleotides for post-transcriptional chemical functionalization |
en_US |
dc.type |
Article |
en_US |
dc.contributor.department |
Dept. of Chemistry |
en_US |
dc.identifier.sourcetitle |
Nature Protocols |
en_US |
dc.publication.originofpublisher |
Foreign |
en_US |