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Generation of miRNA160 and miR166 endogenous Target Mimicry (eTM) transgenic lines in potato and their assessment against Phytophthora interactions.

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dc.contributor.advisor BANERJEE, ANJAN K. en_US
dc.contributor.author K S, SUPREETH en_US
dc.date.accessioned 2015-05-05T09:36:31Z
dc.date.available 2015-05-05T09:36:31Z
dc.date.issued 2015-05 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/446
dc.description.abstract One of the great challenges for researchers is to overcome the late blight disease of potato caused by the oomycete pathogen, Phytophthora infestans. Recent studies suggest involvement of several microRNAs during plant-pathogen interaction. microRNAs 160 (miR160) and miR166 are highly conserved miRNAs among different plant species that are known to play various developmental roles. Studies in our lab indicate involvement of miR160 and miR166 during potato – Phytophthora interaction. In order to further investigate the role of these miRNAs in plant immunity, we generated knockdown lines of miR160 and miR166 using endogenous target mimicry (eTM) approach. miR160, miR166 and their target genes were successfully detected in our model system Solanum tuberosum spp. Desiree. Using Agrobacterium-mediated transformation technique, we successfully generated endogenous target mimicry (eTM) lines for miR160 and miR166. Representative eTM lines of both these miRNAs have been confirmed by RT-PCR analysis. To assess the level of knockdown in these lines, real-time PCR based approach was attempted. Our results indicate successful reduction in levels of miR160 by ~ 66% in eTM160 clone-1 and by ~91% in eTM160 clone-2, whereas reduction of miR166 level was ~ 96% in eTM166 clone 1 and by ~91% in eTM166 clone 2. All the four clones were subsequently transferred to soil and were gradually hardened. These lines are presently grown in growth chamber conditions and are being subjected to Phytophthora infection to understand their role in immunity. en_US
dc.description.sponsorship IISER Pune, Inspire. en_US
dc.language.iso en en_US
dc.subject 2015
dc.subject miRNA en_US
dc.subject Phytophthora infestans en_US
dc.subject Plant immunity en_US
dc.subject qPCR en_US
dc.subject Late blight en_US
dc.title Generation of miRNA160 and miR166 endogenous Target Mimicry (eTM) transgenic lines in potato and their assessment against Phytophthora interactions. en_US
dc.title.alternative Role of miRNAs in plant pathogen interaction. en_US
dc.type Thesis en_US
dc.type.degree BS-MS en_US
dc.contributor.department Dept. of Biology en_US
dc.contributor.registration 20091087 en_US


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  • MS THESES [1705]
    Thesis submitted to IISER Pune in partial fulfilment of the requirements for the BS-MS Dual Degree Programme/MSc. Programme/MS-Exit Programme

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