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Exploring Sex Difference in Epigenetic and Transcription Regulatory MechanisMS Underlying Neuroglial and Behavioral Plasticity in the Prenatal Stress-Induced Mouse Model of Depression

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dc.contributor.advisor Kumar, Arvind en_US
dc.contributor.author MUSTHAFA U K, THASNEEM en_US
dc.date.accessioned 2020-06-22T08:35:49Z
dc.date.available 2020-06-22T08:35:49Z
dc.date.issued 2020-06 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/4820
dc.description.abstract Depression is a debilitating neuropsychiatric disorder predicted to be the leading cause of disability by 2035 as per WHO, yet the underlying molecular mechanisms are unknown. Prenatal or gestational stress has a major contribution to many neuropsychiatric disorders, including depression. Here we have tried to study the effects of prenatal stress on the offspring’s hippocampus in late adulthood. We used animal behavior paradigms to assess the anxiety and depression like phenotypes and indeed we were able to observe mood disorder phenotype in male offsprings and not in females. Our intense real time PCR studies to understand the epigenetic regulators involved in underlying neurobehavioural changes, specifically histone H3 and H4 lysine demethylases and lysine methyl trasferases. Western blot studies for assessing global changes in methylation specific epigenetic marks led us to uncover significant change in H3K4me3, H3K9me1 and H4K20me1, along with change in the transcript levels of demethylases/methyltransferases responsible for demethylating/methylating histone lysines. Previous lab microarray data had indicated alteration in some forkhead family transcription factors in hippocampus in sex biased manner. Here we successfully validated Foxb2 and Foxh1 in gender-specific manner. Further, our ChIP-qPCR analysis showed differential enrichment of Foxb2 on promoters of Sox2, Nestin and Synaptotagmin genes, the neurogenesis and neuroplasticity markers. Irrespective of the enrichment at the promoter region of genes that control neurogenesis, these genes were found upregulated in female while unaltered in male hippocampus. So from these novel data it is difficult to elucidate role of FoxB2 in neurogenesis; more studies are required to get better insight. en_US
dc.description.sponsorship CCMB Hyderabad en_US
dc.language.iso en en_US
dc.subject Depression en_US
dc.subject Epigenetics en_US
dc.subject Transcription Regulation en_US
dc.subject Histone demethylase en_US
dc.subject Histone methyl tarnsferase en_US
dc.subject Forkhead en_US
dc.subject 2020 en_US
dc.title Exploring Sex Difference in Epigenetic and Transcription Regulatory MechanisMS Underlying Neuroglial and Behavioral Plasticity in the Prenatal Stress-Induced Mouse Model of Depression en_US
dc.type Thesis en_US
dc.type.degree BS-MS en_US
dc.contributor.department Dept. of Biology en_US
dc.contributor.registration 20141107 en_US


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  • MS THESES [1705]
    Thesis submitted to IISER Pune in partial fulfilment of the requirements for the BS-MS Dual Degree Programme/MSc. Programme/MS-Exit Programme

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