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Process for Determining Enzyme Activity in a Cell by Activity-based Reporter Gene Technology (AbRGT)

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dc.date.accessioned 2020-08-20T10:08:47Z
dc.date.available 2020-08-20T10:08:47Z
dc.date.issued 2019-11 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/4972
dc.description.abstract Methods and materials for specific imaging of active enzyme in a live or intact cell are disclosed. The enzyme of interest tagged to reporter protein (donor) is exogenously expressed in a cell. The conversion of proenzyme to active enzyme (containing reporter protein) is achieved upon applying an appropriate stimulus to the target cells. The activated enzyme is labelled with an activity-based probe carrying a fluorophore (acceptor). The covalent labelling of active enzyme by the activity-based probe creates a FRET pair which provides the opportunity to exquisitely image the function of an “active enzyme†. This method is used for specific imaging of the function of active caspase-3,-7,-8,-9 and cathepsin-B and also for profiling of inhibitors of caspases and cathepsin B. en_US
dc.language.iso en en_US
dc.subject Active enzyme en_US
dc.subject Specific imaging en_US
dc.subject 2019 en_US
dc.title Process for Determining Enzyme Activity in a Cell by Activity-based Reporter Gene Technology (AbRGT) en_US
dc.type Patent en_US
dc.publisher.department Dept. of Biology en_US
dc.publisher.department Dept. of Chemistry en_US
dc.assignee Indian Institute of Science Education and Research Pune en_US
dc.identifier.patentnumber US20190338373A1 en_US
dc.application.number US16401906A en_US
dc.date.application 2019-05 en_US
dc.publication.kindcode A1 en_US
dc.publication.country IN en_US
dc.contributor.inventor BRITTO, SANDANARAJ S. en_US
dc.contributor.inventor BATHLA, PUNITA en_US


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