Responsive fluorescent nucleotides serve as efficient substrates to probe terminal uridylyl transferase

dc.contributor.author	GEORGE, JERRIN THOMAS	en_US
dc.contributor.author	SRIVATSAN, SEERGAZHI G.	en_US
dc.date.accessioned	2020-09-28T08:23:13Z
dc.date.available	2020-09-28T08:23:13Z
dc.date.issued	2020-10	en_US
dc.identifier.citation	Chemical Communications, 56(82), 12319-12322.	en_US
dc.identifier.issn	1359-7345	en_US
dc.identifier.issn	1364-548X	en_US
dc.identifier.uri	http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/5076
dc.identifier.uri	https://doi.org/10.1039/D0CC05092J	en_US
dc.description.abstract	We repurposed a terminal uridylyl transferase enzyme to site-specifically label RNA with microenvironment sensing fluorescent nucleotide mimics, which in turn provided direct read-outs to estimate the binding affinities of the enzyme to RNA and nucleotide substrates. This enzyme–probe system provides insights into the catalytic cycle, and can facilitate the development of discovery platforms to identify robust enzyme inhibitors.	en_US
dc.language.iso	en	en_US
dc.publisher	Royal Society of Chemistry	en_US
dc.subject	Chemistry	en_US
dc.subject	2020	en_US
dc.subject	2020-SEP-WEEK5	en_US
dc.subject	TOC-SEP-2020	en_US
dc.title	Responsive fluorescent nucleotides serve as efficient substrates to probe terminal uridylyl transferase	en_US
dc.type	Article	en_US
dc.contributor.department	Dept. of Chemistry	en_US
dc.identifier.sourcetitle	Chemical Communications	en_US
dc.publication.originofpublisher	Foreign	en_US