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Effects of arsenite stress on growth and proteome of Klebsiella pneumoniae

Show simple item record Daware, Vandana en_US Kesavan, Suresh en_US Patil, Rajendra en_US NATU, ARVIND en_US Kumar, Ameeta en_US Kulkarni, Mahesh en_US Gade, Wasudev en_US 2020-10-19T04:12:47Z 2020-10-19T04:12:47Z 2012-03 en_US
dc.identifier.citation Journal of Biotechnology, 158(1-2), 8-16. en_US
dc.identifier.issn 0168-1656 en_US
dc.identifier.issn 1873-4863 en_US
dc.identifier.uri en_US
dc.description.abstract In the present study an arsenite, As(III), tolerating bacterium, MR4, was isolated from Mulla River Pune, India, capable of reducing arsenate to arsenite and identified as Klebsiella pneumoniae (HQ857583). Comparative proteomic analysis using two-dimensional gel electrophoresis (2-DGE) and matrix assisted laser desorption ionization-time of flight-time of flight (MALDI-TOF/TOF) was used to monitor the proteins undergoing changes in expression levels under 2.5 mM As(III) stress. The 2-DGE proteome map has shown that 60 proteins were differentially expressed under As(III) stress, of which 39 proteins were successfully identified with a MASCOT score greater than 70 (p < 0.05). Among the identified proteins, membrane transport/binding proteins, porins, and amino acid metabolism enzymes were down-regulated while stress responsive proteins and antioxidant enzymes were up-regulated. Proteins involved in carbohydrate metabolism, particularly those in pentose phosphate pathway were also up-regulated while those involved in pyruvate metabolism were down-regulated. However, proteins involved in glycolysis and tricarboxylic acid cycle showed a mixed regulation response. These findings provide new insights into the probable mechanisms by which K. pneumoniae (HQ857583) could be adapting to As(III) stress. en_US
dc.language.iso en en_US
dc.publisher Elsevier B.V. en_US
dc.subject Klebsiella pneumonia en_US
dc.subject Arsenite stress en_US
dc.subject Two dimensional gel electrophoresis (2-DGE) en_US
dc.subject MALDI-TOF-TOF en_US
dc.subject 2012 en_US
dc.title Effects of arsenite stress on growth and proteome of Klebsiella pneumoniae en_US
dc.type Article en_US
dc.contributor.department Dept. of Chemistry en_US
dc.identifier.sourcetitle Journal of Biotechnology en_US
dc.publication.originofpublisher Foreign en_US

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