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Role of Lamin A and Telomerase in DNA Damage Response

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dc.contributor.advisor SENGUPTA, KUNDAN en_US
dc.contributor.author SUSHANTH, SISHIL en_US
dc.date.accessioned 2016-05-05T05:16:01Z
dc.date.available 2016-05-05T05:16:01Z
dc.date.issued 2016-05 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/604
dc.description.abstract Lamins are intermediate filament proteins that maintain structural and mechanical integrity of the nucleus. Lamins are important in maintenance of the epigenetic landscape, DNA replication and mechanotransduction. Lamins have also been implicated in DNA damage response. Telomerase – a reverse transcriptase enzyme expressed in germ cells and deregulated in cancers, protects transformed cells against DNA damage. In this study, we attempted to understand the coregulatory role of Lamin A and Telomerase in DNA Damage response. Down regulation of Lamin A using shRNA in HT1080 (Telomerase positive) and U2OS (Telomerase negative) cells led to significant decrease in 53BP1 (a key regulator of NHEJ mediated DNA damage repair pathway) foci volumes and changes in foci numbers upon DNA damage induction using Cisplatin. Lamin A downregulated cells showed an enrichment of the 53BP1 foci at the nuclear periphery. HT1080 cells stably overexpressing Telomerase showed comparable levels of basal DNA damage despite being aneuploid and displayed significant downregulation of B-type Lamins. This data suggests a potential cross talk between Telomerase and Lamins in regulating DNA damage response. en_US
dc.language.iso en en_US
dc.subject 2016
dc.subject Lamins en_US
dc.subject Telomerase en_US
dc.subject DNA Damage en_US
dc.subject 53BP1 en_US
dc.title Role of Lamin A and Telomerase in DNA Damage Response en_US
dc.type Thesis en_US
dc.type.degree BS-MS en_US
dc.contributor.department Dept. of Biology en_US
dc.contributor.registration 20111024 en_US


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  • MS THESES [1705]
    Thesis submitted to IISER Pune in partial fulfilment of the requirements for the BS-MS Dual Degree Programme/MSc. Programme/MS-Exit Programme

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