dc.contributor.author |
Deshmukh, Prachi |
en_US |
dc.contributor.author |
Markande, Shubha |
en_US |
dc.contributor.author |
Fandade, Vikas |
en_US |
dc.contributor.author |
RAMTIRTHA, YOGENDRA |
en_US |
dc.contributor.author |
MADHUSUDHAN, M. S. |
en_US |
dc.contributor.author |
Joseph, Jomon |
en_US |
dc.date.accessioned |
2021-08-27T11:21:37Z |
|
dc.date.available |
2021-08-27T11:21:37Z |
|
dc.date.issued |
2021-06 |
en_US |
dc.identifier.citation |
Biochemical and Biophysical Research Communications, 556, 45-52. |
en_US |
dc.identifier.issn |
0006-291X |
en_US |
dc.identifier.uri |
http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/6210 |
|
dc.identifier.uri |
https://doi.org/10.1016/j.bbrc.2021.03.140 |
en_US |
dc.description.abstract |
Micro-RNA mediated suppression of mRNA translation represents a major regulatory mode of post-transcriptional gene expression. Recently, the nucleoporin Nup358 was shown to interact with AGO protein, a key component of miRNA-induced silencing complex (miRISC), and facilitate the coupling of miRISC with target mRNA. Previous results suggested that SUMO-interacting motifs (SIMs) present on Nup358 mediate interaction with AGO protein. Here we show that Nup358-SIM has multiple interacting regions on AGO2, specifically within the N, PAZ and MID domains, with an affinity comparable to SIM-SUMO1 interaction. The study also unraveled specific residues involved in the interaction of AGO2 with miRNA-loading components such as Dicer and HSP90. Collectively, the results support the conclusion that multiple SIMs contribute to the association of Nup358 with AGO2. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Elsevier B.V. |
en_US |
dc.subject |
miRNA |
en_US |
dc.subject |
Nup358 |
en_US |
dc.subject |
SUMO-interaction motif |
en_US |
dc.subject |
AGO2 |
en_US |
dc.subject |
Dicer |
en_US |
dc.subject |
Hsp90 |
en_US |
dc.subject |
2021-AUG-WEEK4 |
en_US |
dc.subject |
2021 |
en_US |
dc.title |
The miRISC component AGO2 has multiple binding sites for Nup358 SUMO-interacting motif |
en_US |
dc.type |
Article |
en_US |
dc.contributor.department |
Dept. of Biology |
en_US |
dc.identifier.sourcetitle |
Biochemical and Biophysical Research Communications |
en_US |
dc.publication.originofpublisher |
Foreign |
en_US |