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Site Selective Modification of Peptides and Proteins

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dc.contributor.advisor GOPI, HOSAHUDYA N. en_US
dc.contributor.author KUMAR, MANISH en_US
dc.date.accessioned 2021-09-06T06:08:59Z
dc.date.available 2021-09-06T06:08:59Z
dc.date.issued 2021-09 en_US
dc.identifier.citation 59 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/6246
dc.description.abstract The diazo-coupling reactions of arenediazonium salts with Tyrosine containing peptides have shown a good selectivity to the Tyrosine residues over other competitive amino acids. In addition to that arenediazonium salts are highly reactive, which allows diazo-coupling reaction to happen even at very mild conditions. So, to develop a spectrophotometric detection of the Tyrosine containing peptides, we have synthesized a series of azo-dyes with Tyrosine and calculated their molar absorptivity coefficients. We observed Tyrosine and other aromatic amino acids containing peptides shows higher selectivity of diazocoupling reaction on Tyrosine residue giving yield of 70-80%. We also studied the relative reactivity of different arenediazonium salts with Tyrosine by monitoring the reaction kinetics by using a UV-Vis spectrophotometer. en_US
dc.description.sponsorship DST, INSPIRE en_US
dc.language.iso en en_US
dc.subject Bioconjugation, Diazocoupling, Selective modification, Tyrosine selective en_US
dc.subject Bioconjugation en_US
dc.subject Diazocoupling en_US
dc.subject Selective modificatio en_US
dc.subject Selective modificatio en_US
dc.title Site Selective Modification of Peptides and Proteins en_US
dc.type Thesis en_US
dc.type.degree BS-MS en_US
dc.contributor.department Dept. of Chemistry en_US
dc.contributor.registration 20161012 en_US


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  • MS THESES [1705]
    Thesis submitted to IISER Pune in partial fulfilment of the requirements for the BS-MS Dual Degree Programme/MSc. Programme/MS-Exit Programme

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