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Redox Biochemistry of Hydrogen Sulfide Biogenesis and Oxidation

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dc.contributor.advisor Banerjee, Ruma en_US
dc.contributor.author GUHA, ARKAJIT en_US
dc.date.accessioned 2021-09-09T03:59:59Z
dc.date.available 2021-09-09T03:59:59Z
dc.date.issued 2021-07 en_US
dc.identifier.citation 42 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/6258
dc.description.abstract Hydrogen sulfide (H2S) is a gaseous molecule which can act as an inorganic substrate for energy production. On the other hand, high concentrations of H2S can act as a poison by inhibiting cellular respiration, but the mechanism of H2S clearance when respiration is inhibited is unclear. Sulfide quinone oxidoreductase (SQOR) catalyzes the initial step of the mitochondrial sulfide oxidation pathway by oxidizing H2S to a persulfide and transferring the electrons to coenzyme Q (CoQ). In this study, we have investigated how competition between SQOR and other enzymes for use of the CoQ pool modulates the capacity for H2S clearance. Monitoring the oxygen consumption rate by the electron transport chain, we found that cells with compromised complex I or complex II activity exhibit enhanced or decreased ability to clear H2S, respectively, suggesting that both complexes are significant modulators of SQOR activity. When complex IV-dependent respiration was inhibited, complex II was found to work in reverse, oxidizing CoQH2 to CoQ by using fumarate as an alternate electron acceptor and increasing sulfide oxidation capacity. We also demonstrated that the malate aspartate shuttle plays an important role in providing fumarate to sustain SQOR activity under these conditions. Complex II-dependent SQOR activity could be a mechanism by which H2S acts as a protective agent during reperfusion following ischemia, attenuating injury. In related studies, we investigated an altered response to H2S following knock down of persulfide dioxygenase (ETHE1), the second enzyme in the mitochondrial sulfide oxidation pathway. Finally, modulation of cystathionine beta synthase activity by select metabolites was probed en_US
dc.description.sponsorship University of Michigan, Ann Arbor; Kishore Vaigyanik Protsahan Yojana (KVPY) en_US
dc.language.iso en en_US
dc.subject Hydrogen Sulfide en_US
dc.subject Reverse Electron Transfer en_US
dc.title Redox Biochemistry of Hydrogen Sulfide Biogenesis and Oxidation en_US
dc.title.alternative Hydrogen Sulfide induced reverse electron transfer en_US
dc.type Article en_US
dc.type Journal en_US
dc.type Dataset en_US
dc.type Image en_US
dc.type Software en_US
dc.type Thesis en_US
dc.type.degree BS-MS en_US
dc.contributor.department Dept. of Chemistry en_US
dc.contributor.registration 20161054 en_US


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  • MS THESES [1705]
    Thesis submitted to IISER Pune in partial fulfilment of the requirements for the BS-MS Dual Degree Programme/MSc. Programme/MS-Exit Programme

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