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Deleterious role of centromeric crossovers in chromosomal segregation during meiosis

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dc.contributor.advisor NAMBIAR, MRIDULA en_US
dc.contributor.author SARKAR, SNEHA en_US
dc.date.accessioned 2021-09-23T11:12:20Z
dc.date.available 2021-09-23T11:12:20Z
dc.date.issued 2021-08 en_US
dc.identifier.citation 37 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/6275
dc.description.abstract Crossover formation (CO) during meiotic recombination is one of the important processes that provides right amount of tension for the homologous chromosomes to properly segregate in the first meiotic division (MI). CO formation is necessary in meiosis, yet regions near centromere (pericentric regions) have mechanisms to repress them. Earlier studies have shown that COs that are too close to the centromere, leads to meiotic chromosome mis-segregation, which may further result in aneuploid gametes and eventually aneuploid or dead zygote. Although the harmful effects of COs near the centromeres are well known, the different kinds of mis-segregations that it gives rise to and the mechanisms behind them have still not been well discussed. Here, we have used live cell imaging in mutants proficient in centromeric recombination to understand how pericentric crossovers can lead to defects in chromosomal segregation during meiosis in fission yeast, Schizosaccharomyces pombe. Live cell imaging provides a more effective approach than using viability assays to address the basis of chromosomal mis-segregation resulting from pericentric crossovers. An assay was designed to determine centromeric crossover (C-CO) frequency and classify the different segregation patterns in the mutants into meiosis I or meiosis II mis-segregation events. Lastly, a strategy has been planned to further look into the mechanism behind such mis-segregations. en_US
dc.language.iso en en_US
dc.subject Centromeric crossover en_US
dc.subject Chromosomal mis-segregation en_US
dc.subject Schizosaccharomyces pombe en_US
dc.subject Spore autonomous promoter en_US
dc.subject Meiosis I non-disjunction en_US
dc.subject Meiosis II non-disjunction en_US
dc.subject Premature separation of sister chromatids en_US
dc.title Deleterious role of centromeric crossovers in chromosomal segregation during meiosis en_US
dc.type Thesis en_US
dc.type.degree BS-MS en_US
dc.contributor.department Dept. of Biology en_US
dc.contributor.registration 20161195 en_US


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  • MS THESES [1705]
    Thesis submitted to IISER Pune in partial fulfilment of the requirements for the BS-MS Dual Degree Programme/MSc. Programme/MS-Exit Programme

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