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Nup93 and CTCF modulate spatiotemporal dynamics and function of the HOXA gene locus during differentiation

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dc.contributor.author LABADE, AJAY S. en_US
dc.contributor.author SALVI, ADWAIT en_US
dc.contributor.author KAR, SASWATI en_US
dc.contributor.author KARMODIYA, KRISHANPAL en_US
dc.contributor.author SENGUPTA, KUNDAN en_US
dc.date.accessioned 2022-02-25T10:24:45Z
dc.date.available 2022-02-25T10:24:45Z
dc.date.issued 2021-12 en_US
dc.identifier.citation Journal of Cell Science, 134(23), jcs259307. en_US
dc.identifier.issn 0021-9533 en_US
dc.identifier.issn 1477-9137 en_US
dc.identifier.uri https://doi.org/10.1242/jcs.259307 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/6595
dc.description.abstract Nucleoporins regulate nuclear transport and are also involved in DNA damage, repair, cell cycle, chromatin organization and gene expression. Here, we studied the role of nucleoporin Nup93 and the chromatin organizer CTCF in regulating expression of the HOXA gene locus during differentiation. ChIP sequencing revealed a significant overlap between Nup93 and CTCF peaks. Interestingly, Nup93 and CTCF are associated with the 3′ and 5′ HOXA genes, respectively. Depletions of Nup93 and CTCF antagonistically modulate expression levels of 3′ and 5′ HOXA genes in the undifferentiated human NT2/D1 cell line. Nup93 also regulates the localization of the HOXA gene locus, which disengages from the nuclear periphery upon Nup93 but not CTCF depletion, consistent with its upregulation. The dynamic association of Nup93 and CTCF with the HOXA locus during differentiation correlates with its spatial positioning and expression. Whereas Nup93 tethers the HOXA locus to the nuclear periphery, CTCF potentially regulates looping of the HOXA gene cluster in a temporal manner. In summary, Nup93 and CTCF complement one another in modulating the spatiotemporal dynamics and function of the HOXA gene locus during differentiation. en_US
dc.language.iso en en_US
dc.publisher The Company of Biologists Ltd en_US
dc.subject Nucleoporins en_US
dc.subject Nup93 en_US
dc.subject CTCF en_US
dc.subject CBS en_US
dc.subject HOXA en_US
dc.subject Chromatin en_US
dc.subject Retinoic acid en_US
dc.subject Differentiation en_US
dc.subject 2022-FEB-WEEK4 en_US
dc.subject TOC-FEB-2022 en_US
dc.subject 2021 en_US
dc.title Nup93 and CTCF modulate spatiotemporal dynamics and function of the HOXA gene locus during differentiation en_US
dc.type Article en_US
dc.contributor.department Dept. of Biology en_US
dc.identifier.sourcetitle Journal of Cell Science en_US
dc.publication.originofpublisher Foreign en_US


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