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Characterization of Bacterial Chemosensory Protein, FrzE, in Myxococcus xanthus

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dc.contributor.advisor PANANGHAT, GAYATHRI en_US
dc.contributor.author P V, MEENAKSHI en_US
dc.date.accessioned 2022-05-17T04:47:37Z
dc.date.available 2022-05-17T04:47:37Z
dc.date.issued 2022-05
dc.identifier.citation 53 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/6960
dc.description.abstract The life cycle processes like fruiting body formation and vegetative swarming in Myxococcus xanthus, a gram-negative rod-shaped bacterium, require the involvement of controlled movements by gliding motility on surfaces, in response to environmental cues. A chemotaxis pathway, the Frz signal transduction system plays a major role in these cellular reversals. The domains of the proteins encoded by frz genes are homologous to proteins involved in chemotaxis signaling, which facilitates two-component signal transduction. FrzE, a two-component histidine kinase, is a part of the Frz signal transduction system, which autophosphorylates on a conserved histidine residue upon receiving the signal and helps in the further transduction of the signal to downstream components. We characterized the phosphoacceptor domain and the catalytic domain of FrzE, and measured the ATPase activity of FrzE. HPT domain has been purified, crystallized, and diffraction data obtained. ATPase assay shows that FrzE has catalytic activity. Preliminary experiments for cloning the HATPase domain were carried out. Structural analysis of HATPase domains belonging to the HATPase superfamily helped in identifying the conserved residues essential for catalysis. The project is being continued to refine the crystal structure of the HPT domain, purification and interaction studies of the catalytic domain, and further quantification of the ATPase activity of FrzE. en_US
dc.language.iso en en_US
dc.subject Chemosensory en_US
dc.subject FrzE en_US
dc.title Characterization of Bacterial Chemosensory Protein, FrzE, in Myxococcus xanthus en_US
dc.type Thesis en_US
dc.type.degree BS-MS en_US
dc.contributor.department Dept. of Biology en_US
dc.contributor.registration 20171087 en_US


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  • MS THESES [1705]
    Thesis submitted to IISER Pune in partial fulfilment of the requirements for the BS-MS Dual Degree Programme/MSc. Programme/MS-Exit Programme

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