Digital Repository

Pervasive sequence-level variation in the transcriptome of Plasmodium falciparum

Show simple item record

dc.contributor.author DAVE, BRUHAD en_US
dc.contributor.author KANYAL, ABHISHEK en_US
dc.contributor.author MAMATHARANI, D. V. en_US
dc.contributor.author KARMODIYA, KRISHANPAL en_US
dc.date.accessioned 2022-05-31T08:23:01Z
dc.date.available 2022-05-31T08:23:01Z
dc.date.issued 2022-06 en_US
dc.identifier.citation NAR Genomics and Bioinformatics, 4(2), lqac036. en_US
dc.identifier.issn 2631-9268 en_US
dc.identifier.uri https://doi.org/10.1093/nargab/lqac036 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/7002
dc.description.abstract Single-nucleotide variations (SNVs) in RNA, arising from co- and post-transcriptional phenomena including transcription errors and RNA-editing, are well studied in a range of organisms. In the malaria parasite Plasmodium falciparum, stage-specific and non-specific gene-expression variations accompany the parasite's array of developmental and morphological phenotypes over the course of its complex life cycle. However, the extent, rate and effect of sequence-level variation in the parasite's transcriptome are unknown. Here, we report the presence of pervasive, non-specific SNVs in the P. falciparum transcriptome. SNV rates for a gene were correlated to gene length (r≈0.65–0.7) but not to the AT-content of that gene. Global SNV rates for the P. falciparum lines we used, and for publicly available P. vivax and P. falciparum clinical isolate datasets, were of the order of 10–3 per base, ∼10× higher than rates we calculated for bacterial datasets. These variations may reflect an intrinsic transcriptional error rate in the parasite, and RNA editing may be responsible for a subset of them. This seemingly characteristic property of the parasite may have implications for clinical outcomes and the basic biology and evolution of P. falciparum and parasite biology more broadly. We anticipate that our study will prompt further investigations into the exact sources, consequences and possible adaptive roles of these SNVs. en_US
dc.language.iso en en_US
dc.publisher Oxford University Press en_US
dc.subject Biology en_US
dc.subject 2022-MAY-WEEK3 en_US
dc.subject TOC-MAY-2022 en_US
dc.subject 2022 en_US
dc.title Pervasive sequence-level variation in the transcriptome of Plasmodium falciparum en_US
dc.type Article en_US
dc.contributor.department Dept. of Biology en_US
dc.identifier.sourcetitle NAR Genomics and Bioinformatics en_US
dc.publication.originofpublisher Foreign en_US


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search Repository


Advanced Search

Browse

My Account