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A multi-omics analysis reveals that the lysine deacetylase ABHD14B influences glucose metabolism in mammals

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dc.contributor.author RAJENDRAN, ABINAYA en_US
dc.contributor.author SOORY, AMARENDRANATH en_US
dc.contributor.author KHANDELWAL, NEHA en_US
dc.contributor.author RATNAPARKHI, GIRISH S. en_US
dc.contributor.author KAMAT, SIDDHESH S. en_US
dc.date.accessioned 2022-06-16T04:23:35Z
dc.date.available 2022-06-16T04:23:35Z
dc.date.issued 2022-07 en_US
dc.identifier.citation Journal of Biological Chemistry, 298(7), 2022, 102128. en_US
dc.identifier.issn 0021-9258 en_US
dc.identifier.uri https://doi.org/10.1016/j.jbc.2022.102128 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/7092
dc.description.abstract The sirtuins and histone deacetylases are the best characterized members of the lysine deacetylase (KDAC) enzyme family. Recently, we annotated the “orphan” enzyme ABHD14B (α/β-hydrolase domain containing protein # 14B) as a novel KDAC, showed this enzyme’s ability to transfer an acetyl-group from protein lysine residue(s) to coenzyme-A (CoA) to yield acetyl-CoA, expanding the repertoire of this enzyme family. However, the role of ABHD14B in metabolic processes is not fully elucidated. Here, we investigated the role of this enzyme using mammalian cell knockdowns in a combined transcriptomics, and metabolomics analysis. We found from these complementary experiments in vivo, that the loss of ABHD14B results in significantly altered glucose metabolism, specifically the decreased flux of glucose through glycolysis and the citric acid cycle. Further, we show that depleting hepatic ABHD14B in mice, also results in defective systemic glucose metabolism, particularly during fasting. Taken together, our findings illuminate the important metabolic functions that the KDAC ABHD14B plays in mammalian physiology, and poses new questions regarding the role of this hitherto cryptic metabolism-regulating enzyme. en_US
dc.language.iso en en_US
dc.publisher Elsevier B.V. en_US
dc.subject ABHD14B en_US
dc.subject Lysine deacetylase en_US
dc.subject Transcriptomics en_US
dc.subject Metabolomics en_US
dc.subject Glucose Metabolism en_US
dc.subject 2022-JUN-WEEK3 en_US
dc.subject TOC-JUN-2022 en_US
dc.subject 2022 en_US
dc.title A multi-omics analysis reveals that the lysine deacetylase ABHD14B influences glucose metabolism in mammals en_US
dc.type Article en_US
dc.contributor.department Dept. of Biology en_US
dc.identifier.sourcetitle Journal of Biological Chemistry en_US
dc.publication.originofpublisher Foreign en_US


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