dc.contributor.author |
KULKARNI, BHAGYASHREE |
en_US |
dc.contributor.author |
MALHOTRA, MEHAK |
en_US |
dc.contributor.author |
JAYAKANNAN, MANICKAM |
en_US |
dc.date.accessioned |
2022-06-24T10:42:13Z |
|
dc.date.available |
2022-06-24T10:42:13Z |
|
dc.date.issued |
2019-11 |
en_US |
dc.identifier.citation |
ACS Applied Polymer Materials, 1(12), 3375–3388. |
en_US |
dc.identifier.issn |
2637-6105 |
en_US |
dc.identifier.uri |
https://doi.org/10.1021/acsapm.9b00800 |
en_US |
dc.identifier.uri |
http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/7185 |
|
dc.description.abstract |
The present investigation reports enzyme-biodegradable perylenebisimide (PBI)-tagged polycaprolactone (PCL) block copolymers, and their aqueous nanoassemblies were employed as probes for intracellular bio-imaging in cancer cells. Bishydroxyl functionalized PBI initiator was tailor-made, and it was employed as initiator for the ring opening polymerization (ROP) methodology to make PBI-tagged tert-butyl ester-substituted polycaprolactone (PBI-BPCLx) block copolymers. The deprotection of these copolymers yielded carboxylic acid-substituted PBI-CPCLx amphiphilic block copolymers. The carboxylic blocks were self-assembled to produce stable red-fluorescent nanoparticles of <150 nm in size in aqueous medium with fluorescent quantum yield of ϕ = 0.25–0.30 suitable for bio-imaging application. In vitro studies confirmed that the aliphatic polyester backbone in the PBI-CPCLx polymer nanoparticles was readily biodegradable by lysosomal enzymes under physiological conditions. Dynamic light scattering, gel permeation chromatography, photophysical studies, and MALDI-TOF-MS analysis provided evidence of the enzymatic biodegradation. Cytotoxicity studies revealed that the PBI-CPCLx nanoparticles were highly biocompatible toward both cervical cancer and breast cancer cell lines up to a concentration of 100 μg/mL. Confocal microscopy analysis confirmed the uptake and accumulation of red-fluorescent PBI-CPCLx polymer nanoparticles in the perinuclear environment of the cells. The present approach puts forward a PBI-PCL block copolymer design as enzyme-responsive red-fluorescent nanoprobes for bio-imaging in cancer and normal cells. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
American Chemical Society |
en_US |
dc.subject |
Cells |
en_US |
dc.subject |
Copolymers |
en_US |
dc.subject |
Fluorescence |
en_US |
dc.subject |
Nanoparticles |
en_US |
dc.subject |
Polymers |
en_US |
dc.subject |
2019 |
en_US |
dc.title |
Perylene-Tagged Polycaprolactone Block Copolymers and Their Enzyme-Biodegradable Fluorescent Nanoassemblies for Intracellular Bio-imaging in Cancer Cells |
en_US |
dc.type |
Article |
en_US |
dc.contributor.department |
Dept. of Chemistry |
en_US |
dc.identifier.sourcetitle |
ACS Applied Polymer Materials |
en_US |
dc.publication.originofpublisher |
Foreign |
en_US |