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Role of Caspar and Ter94 in Early Drosophila Development

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dc.contributor.advisor RATNAPARKHI, GIRISH
dc.contributor.author PAWAR, SHRUTI
dc.date.accessioned 2023-05-16T05:32:08Z
dc.date.available 2023-05-16T05:32:08Z
dc.date.issued 2023-05
dc.identifier.citation 43 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/7865
dc.description.abstract Caspar in Drosophila is an ortholog of mammalian Fas Associated Factor 1 (FAF1) and its domain structure is suggestive of the function of Casp protein being conserved between fruit flies and humans. It has been studied in the context of fly immunity; however, it has been shown in the lab to have a developmental role as well. Casp expresses maternally. It interacts with ER-associated degradation protein Ter94 (Transitional endoplasmic reticulum 94). Casp and Ter94 are required for cell division in the early embryo and their loss leads to nuclear and cytoskeletal defects. Also, developmental progression of casplof embryos stalls before gastrulation which is an important step in Drosophila embryogenesis. Further, Casp and Ter94 have been shown to play a role in pole cell formation. Based on its domain structure, we hypothesize that Casp might be functioning as an adapter for the ER-associated degradation pathway, helping to target unfolded proteins to the ubiquitin-proteasomal system, by connecting ubiquitinated proteins (bound by N-terminal UBA domain) to Ter94 (possibly bound by C-terminal UBX domain). We choose Me31b (Maternal expression at 31B), a protein involved in the global repression of maternal mRNAs during the maternal to zygotic transition (MZT) in the early Drosophila embryo, as a target protein to check if Casp alone or in conjunction with Ter94 has a role in degrading proteins during the MZT. In our study, data is suggestive of Ter94 not playing a role in Me31b degradation and whether Casp plays a role in the same remains an open question. en_US
dc.language.iso en en_US
dc.subject Caspar en_US
dc.subject Maternal Degradation during MZT en_US
dc.subject Genetic Interaction between Ter94 and Caspar en_US
dc.subject Imaging en_US
dc.subject Maternal Genetics en_US
dc.subject Early Embryo Development en_US
dc.subject Drosophila en_US
dc.title Role of Caspar and Ter94 in Early Drosophila Development en_US
dc.type Thesis en_US
dc.description.embargo One Year en_US
dc.type.degree BS-MS en_US
dc.contributor.department Dept. of Biology en_US
dc.contributor.registration 20181209 en_US


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  • MS THESES [1705]
    Thesis submitted to IISER Pune in partial fulfilment of the requirements for the BS-MS Dual Degree Programme/MSc. Programme/MS-Exit Programme

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