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Production of industrially relevant proteins using cell-wall deficient cells

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dc.contributor.advisor Claessen, Dennis
dc.contributor.author PATWARDHAN, CHINMAY
dc.date.accessioned 2023-05-18T08:52:51Z
dc.date.available 2023-05-18T08:52:51Z
dc.date.issued 2023-04
dc.identifier.citation 65 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/7902
dc.description.abstract Over the last few decades, E. coli cells have been increasingly used in industrial biotechnology to produce products of high economic value. This includes enzymes, therapeutics, and dietary supplements. However, currently used E. coli systems have drawbacks, making them sub-optimal for producing certain types of products. L-form E. coli could target these issues and be established as an industrially viable strain for specific commercial products. L-forms are cell wall deficient strains of bacteria that can be created using mutations, spacial constraints, or antibiotic treatments. Their property of L-form switching could provide robustness in industrial settings. This project deals with understanding the properties of an E. coli L-form strain and how it responds to standard DNA manipulation and transformation techniques. The project also looks at this strain’s ability to produce simple fluorescent proteins and goes on to scale up to more complex proteins like enzymes. Using two enzymes, ɑ-amylase and β-galactosidase, we show that the L-form strain can also produce functional enzymes. We were able to come up with a new protocol for reliably transforming L-form E. coli and were also able to determine the optimal conditions for protein expression on solid media. Scaling up experimentation with bioreactors and testing the enzymes in a liquid assay format would allow for a better understanding of L-form E. coli. Creating a stable L-form from an industrial strain would also aid in comparing protein production between various strains. en_US
dc.description.sponsorship Leiden University en_US
dc.language.iso en en_US
dc.subject L-form en_US
dc.subject Cell wall deficient en_US
dc.subject Industrial Proteins en_US
dc.subject Enzyme Assays en_US
dc.subject Secretion Tags en_US
dc.title Production of industrially relevant proteins using cell-wall deficient cells en_US
dc.type Article en_US
dc.type Journal en_US
dc.type Book chapter en_US
dc.type Image en_US
dc.type Thesis en_US
dc.type Dissertation en_US
dc.type Working Paper en_US
dc.description.embargo One Year en_US
dc.type.degree BS-MS en_US
dc.contributor.department Dept. of Biology en_US
dc.contributor.registration 20181198 en_US


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  • MS THESES [1705]
    Thesis submitted to IISER Pune in partial fulfilment of the requirements for the BS-MS Dual Degree Programme/MSc. Programme/MS-Exit Programme

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