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Functional characterization of Arabidopsis orthologs of PAT1 gene in Physcomitrella patens (P. patens)

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dc.contributor.advisor BANERJEE, ANJAN K. en_US
dc.contributor.author JOGDAND, SUKANYA en_US
dc.date.accessioned 2018-04-18T08:07:59Z
dc.date.available 2018-04-18T08:07:59Z
dc.date.issued 2017-03 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/793
dc.description.abstract Plant-specific GRAS proteins are the putative transcription factors that play critical and diverse roles in plant growth and development. GRAS family comprises of seven clades in moss with GRAS domain containing proteins- SCR, SHR, DELLA, LAS/Ls, HAM, SCL and PAT1. Previous studies have showed that, AtPAT1 plays a role in phytochrome signal transduction in A. thaliana, while VaPAT1 is involved in abiotic stress responses in V. amurensis. However, the role of PAT1 have been unexplored in the lower group of plants viz bryophytes. We are interested in understanding the functional role of PAT1 proteins in P. patens. A comprehensive analysis of PpPAL1A and PpPAL1B genes, including gene structure, motif search, sequence alignment, phylogeny, tissue specific expression profile and targeted mutagenesis were performed. The sequence alignment, motif search and phylogenetic tree analyses together confirmed that PpPAL1A and PpPAL1B are the members of the PAT1-branch of the GRAS domain protein family and they showed 82.95% identity at the level of amino acids. Based on the available in silico microarray data, we studied the expression levels of both PpPAL1A and PpPAL1B genes in moss. Interestingly, we observed that PpPAL1A showed constitutive expression in all tissue while PpPAL1B expression was dominated in the sporophytic phase. Our tissue specific gene expression analysis done by semi-quantitative RT-PCR supported the available in silico data, where both the genes showed relatively higher expression level in gametophore compared to protonema. Statistical significance was seen in relative expression levels, in protonema and gametophore tissues for both PpPAL1A and PpPAL1B. The full length coding sequences for PpPAL1A and PpPAL1B were successfully cloned and transformed in moss by Agrobacterium-mediated transformation. Putative transgenic lines are growing in BCDAT media and will be further characterized. Knockout construction of PpΔpal1a and PpΔpal1b vectors and generation of mutant lines by PEG-mediated transformation is currently under progress. These approaches would help us to understand the functional roles of PAT1-like proteins in the transcriptional regulation and modulatory functions of GRAS domain proteins in moss. en_US
dc.language.iso en en_US
dc.subject 2017
dc.subject Biology en_US
dc.subject Arabidopsis orthologs en_US
dc.subject PAT1 gene en_US
dc.subject Physcomitrella patens en_US
dc.title Functional characterization of Arabidopsis orthologs of PAT1 gene in Physcomitrella patens (P. patens) en_US
dc.type Thesis en_US
dc.type.degree BS-MS en_US
dc.contributor.department Dept. of Biology en_US
dc.contributor.registration 20121038 en_US


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  • MS THESES [1705]
    Thesis submitted to IISER Pune in partial fulfilment of the requirements for the BS-MS Dual Degree Programme/MSc. Programme/MS-Exit Programme

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