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Identification and characterization of insulator elements and proteins in Plasmodium falciparum

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dc.contributor.advisor KARMODIYA, KRISHANPAL
dc.contributor.author PRADIP GHADAGE, KALYAN
dc.date.accessioned 2023-05-22T05:00:44Z
dc.date.available 2023-05-22T05:00:44Z
dc.date.issued 2023-05
dc.identifier.citation 54 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/7952
dc.description.abstract Malaria, a life-threatening disease is becoming a major concern on a global scale due to the resistance to the available drug treatment and the lack of an effective vaccine. Plasmodium falciparum, the deadliest Plasmodium species responsible for severe malaria, exhibits antigenic variation through mutually exclusive expression and timely switching of var genes which is responsible for the enhanced pathological complexity during the infection and longevity of the infection. The exact regulatory mechanism of antigenic variation is still unclear, although research to date suggests the potential roles of characteristic positioning of var genes on chromosomes, their subnuclear organization, epigenetic modifications, and non-coding RNAs in the expression of var genes in a mutually exclusive manner. In this study, we are identifying the putative insulator-like proteins in Plasmodium falciparum and investigating the prospect of insulators serving as the possible regulators involved in the mutually exclusive expression of var genes. Through the in-silico approach and wet lab experiments, we have identified the putative insulator-like proteins and validating their role through qPCR followed by overexpression of the proteins of interest. Overall, we are also aiming to identify the putative chromatin architectural proteins in Plasmodium falciparum. Collectively, this study identifies the putative insulator-like proteins in Plasmodium falciparum and provides future directions to characterize their role in var gene expression and 3D genome organization. en_US
dc.language.iso en en_US
dc.subject Plasmodium falciparum en_US
dc.subject insulator proteins en_US
dc.subject genome organization en_US
dc.subject var genes en_US
dc.subject PfHP1 en_US
dc.title Identification and characterization of insulator elements and proteins in Plasmodium falciparum en_US
dc.type Thesis en_US
dc.description.embargo Two Years en_US
dc.type.degree BS-MS en_US
dc.contributor.department Dept. of Biology en_US
dc.contributor.registration 20181175 en_US


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  • MS THESES [1705]
    Thesis submitted to IISER Pune in partial fulfilment of the requirements for the BS-MS Dual Degree Programme/MSc. Programme/MS-Exit Programme

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