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Quantitative analysis of the Arabidopsis shoot apical meristem during floral transition

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dc.contributor.advisor Formosa-Jordan, Pau
dc.contributor.advisor Coupland, George
dc.contributor.author THAMPI, JOHN
dc.date.accessioned 2023-05-22T09:50:49Z
dc.date.available 2023-05-22T09:50:49Z
dc.date.issued 2023-05
dc.identifier.citation 40 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/7974
dc.description.abstract The stem-cell niche at the Shoot Apical Meristem (SAM) in Arabidopsis thaliana undergoes reorganisation during floral transition. This niche is maintained by a well-studied negative feedback loop between WUSCHEL and CLAVATA3, but how this network evolves during floral transition is unknown. In this study, we use confocal imaging and a custom cellular-resolution quantitative image analysis pipeline to study the expression domains of these two genes in the SAM during floral transition using transcriptional reporters of WUSCHEL and CLAVATA3 in wild-type and two mutant lines. We provide a first description of their patterning during the floral transition. We find a transient expansion in the expression domain of WUSCHEL - pointing to a role of the rib zone of the meristem in maintaining stem-cell homeostasis. Our work helps further the current understanding of stem-cell homeostasis in the shoot apical meristem, and of the organisation of the SAM in general. It provides a quantitative framework for future experimental and modelling studies of the system. en_US
dc.description.sponsorship Max Planck Institute for Plant Breeding Research Kishore Vaigyanik Protsahan Yojana en_US
dc.language.iso en en_US
dc.subject Developmental Biology en_US
dc.subject Image Analysis en_US
dc.subject Plant Biology en_US
dc.subject Stem Cell Homeostasis en_US
dc.title Quantitative analysis of the Arabidopsis shoot apical meristem during floral transition en_US
dc.type Thesis en_US
dc.description.embargo One Year en_US
dc.type.degree BS-MS en_US
dc.contributor.department Dept. of Biology en_US
dc.contributor.registration 20181159 en_US


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  • MS THESES [1705]
    Thesis submitted to IISER Pune in partial fulfilment of the requirements for the BS-MS Dual Degree Programme/MSc. Programme/MS-Exit Programme

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