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Developing DILI models in zebrafish for screening of hepatoprotective agents

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dc.contributor.advisor Sachidanandan, Chetana en_US
dc.contributor.author LOKHANDE, LAVANYA en_US
dc.date.accessioned 2018-04-19T05:28:38Z
dc.date.available 2018-04-19T05:28:38Z
dc.date.issued 2017-04 en_US
dc.identifier.uri http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/811
dc.description.abstract Drug Induced Liver injury (DILI) is one of the causes of liver failure. In severe conditions, treatment for DILI is limited to liver transplant. Therefore, there is an urgent need for therapeutics that may counter DILI. Danio rerio, commonly known as zebrafish has emerged as a popular vertebrate model for drug screening. Using zebrafish, as a model system, we have successfully developed embryonic and adult zebrafish DILI models using acetaminophen (APAP) and Isoniazid (INH). Acetaminophen (paracetamol) is a known hepatotoxic molecule, extensively studied in multiple model systems and has become one of the major causes of acute liver failure. Isoniazid is a drug prescribed for Tuberculosis (TB). Since, TB is highly prevalent in India; hepatotoxicity due to INH has become a serious issue in the Indian sub-continent. Using fabp10a as a marker for liver, we have shown by multiple means that both drugs down-regulate the expression of fabp10a thus indicating damage. Other techniques were used to characterize both the DILI models and study their post-damage effects. Using the INH-induced liver-damage model, we have screened 15 small molecules for their hepatoprotective effect and have found one molecule as a potential candidate. These models can be used for further screening of novel small molecules and enable us to understand DILI as well as the mechanisms of small molecules that can revert it. en_US
dc.language.iso en en_US
dc.subject 2017
dc.subject Biology en_US
dc.subject DILI models en_US
dc.subject Zebrafish en_US
dc.subject Hepatoprotective agents en_US
dc.title Developing DILI models in zebrafish for screening of hepatoprotective agents en_US
dc.type Thesis en_US
dc.type.degree BS-MS en_US
dc.contributor.department Dept. of Biology en_US
dc.contributor.registration 20121060 en_US


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  • MS THESES [1705]
    Thesis submitted to IISER Pune in partial fulfilment of the requirements for the BS-MS Dual Degree Programme/MSc. Programme/MS-Exit Programme

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