Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses

VINCHHI, RHEA; JENA, CHINMAYA; MATANGE, NISHAD

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dc.contributor.author	VINCHHI, RHEA	en_US
dc.contributor.author	JENA, CHINMAYA	en_US
dc.contributor.author	MATANGE, NISHAD	en_US
dc.date.accessioned	2024-04-24T05:42:25Z
dc.date.available	2024-04-24T05:42:25Z
dc.date.issued	2023-03	en_US
dc.identifier.citation	Star Protocol, 4(01), 102005.	en_US
dc.identifier.issn	2666-1667	en_US
dc.identifier.uri	https://doi.org/10.1016/j.xpro.2022.102005	en_US
dc.identifier.uri	http://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/8663
dc.description.abstract	Adaptive laboratory evolution (ALE) of bacteria has the potential to provide many insights like revealing novel mechanisms of resistance and elucidating the impact of drug combinations and concentrations on AMR evolution. Here, we describe a step-by-step ALE protocol for the model bacterium Escherichia coli that can be easily adapted to answer questions related to evolution and genetics of AMR in diverse bacteria. Key issues to consider when designing ALE experiments as well as some downstream mutation mapping analyses are described. For complete details on the use and execution of this protocol, please refer to Patel and Matange (2021)1 and Matange et al. (2019).2	en_US
dc.language.iso	en	en_US
dc.publisher	Elsevier B.V.	en_US
dc.subject	Genetics	en_US
dc.subject	Sequencing	en_US
dc.subject	Microbiology	en_US
dc.subject	Model Organisms	en_US
dc.subject	Evolutionary biology	en_US
dc.subject	2023	en_US
dc.title	Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses	en_US
dc.type	Article	en_US
dc.contributor.department	Dept. of Biology	en_US
dc.identifier.sourcetitle	Star Protocol	en_US
dc.publication.originofpublisher	Foreign	en_US